清肺口服液对呼吸道合胞病毒感染人胚肺成纤维细胞TGF-β_1 mRNA基因表达的影响

Effect of Qingfei Oral Liquid on TGF-β_1 mRNA expression of Respiratory Syncytial Virus Infected Human Embryonic Lung Fibroblasts

目的探讨清肺口服液含药血清对呼吸道合胞病毒(RSV)感染的人胚肺成纤维细胞(HELF)转化生长因子-β1(TGF-β1)基因表达的影响。方法 RSV攻击体外培养的HELF细胞后,以清肺口服液含药血清、利巴韦林含药血清及空白血清干预,采用实时荧光定量PCR法测定各组HELF TGF-β1表达变化。结果病毒对照组较正常细胞组TGF-β1下降至1/3,清肺口服液含药血清组可显著升高TGF-β1水平至RSV感染细胞的2倍。利巴韦林含药血清和空白血清作用相似,对TGF-β1表达有一定的影响。结论 RSV感染可使HELF细胞TGF-β1mRNA表达水平显著下降;清肺口服液可调节TGF-β1 mRNA表达,减缓RSV感染后细胞的异常变化,这可能是其抗病毒作用机制之一。

Objective： To study the effect of Qingfei Oral Liquid on expression of TGF-β1 geng in Respiratory Syncytial Virus（RSV） infected Human Embryonic Lung Fibroblasts（HELF）.Methods： HELF cells in vitro cultured infected with RSV were intervened by the Qingfei Oral Liquid medicated serum,the ribavirin medicated serum and the blank serum.The expression variation of the TGF-β1 in the HELF was detected by the Real-time fluorescence quantitative PCR method.Results： Compared with normal cell,TGF-β1 with virus infection was decreased to 1 /3.The TGF-β1 in the Qingfei Oral Liquid medicated serum group was increased to 2 times of that in RSV infected cells.Ribavirin medicated serum group and blank serum group have similar effect.They have certain effect on expression of TGF-β1.Conclusion： The expression level of TGF-β1 mRNA in HELF cells infected with RSV could be decrease significantly.Qingfei Oral Liquid can regulate mRNA expression of TGF-β1 and attenuate the abnormal changes of RSV infected cells.It may be one of the mechanisms of its antiviral effect.