基质细胞衍生因子-1α在诱导骨髓来源细胞到达皮肤创面中的作用

Role of stromal cell-derived factor-1α in inducing recruitment of bone marrow-derived cells to cutaneous wound area

目的 探讨皮肤创面基质细胞衍生因子-1α（stromal cell derived factor-1α,SDF-1α）在诱导骨髓来源细胞（bone marrow derived cells,BMDCs）向创面募集过程中的作用.方法分离培养小鼠BMDCs,并通过CXCR=4（SDF-1d特异性受体）免疫荧光鉴定BMDCs.培养的BMDC8分为正常培养组（BMDCs组）和CXCR4抗体共孵育组（100 ng/ml,anti-CXCR4组）,用CM-DiI标记后分别将各组BMDC8注入全层皮肤缺损伤小鼠模型的尾静脉,同时以注射DMEM/F12无血清培养基作为对照组,观察创面标记的BMDCs数量及创面愈合率. 结果 （1）BMDCs均表达CXCR4;（2）在伤后7和14 d BMDCs组的创面愈合率分别为（41.3±4.6）%和（92.3±2.1）%,显著高于相应时相点对照组[（29.3±2.3）%、（77.3±2.5）%]和anti-CXCR4组[（30.7±4.6）%、（85.7±1.5）%]（P＜0.05）,而anti-CXCR4组的创面愈合率仅在伤后14 d显著高于对照组（P＜0.05）;（3）在伤后7和14 d,BMDCs组创面标记的BMDCs数目分别为（535±84）个/每张切片和（769±124）个/每张切片,显著高于相应时相点的anti-CXCR4组[（335±97）个/每张切片;（521±127）个/每张切片]（P＜0.05）. 结论 BMDCs参与并促进皮肤创面愈合;SDF-1α在诱导BMDCs到达创面过程中有重要作用.

Objective To explore the effect of stromal cell-derived factor-1 α （SDF-1 α） on inducing recruitment of bone marrow-derived cells （BMDCs） to wound area. Methods BMDCs were isolated from bone marrow, cultured with routine method and identified by CXCR4 antibody. Cells cultured with CXCR4 antibody （100 ng,/mL） for 6 hours were labeled with CM-DiI and injected into the tail vein of full-thickness incisional wound model （set as anti-CXCR4 group）. BMDCs labeled with CM-DiI without antibody treatment were injected to the rats in BMDCs group, and rats were injected with DMEM/F12 serum-free medium in the control group. The quantity of labeled BMDCs at the wound site and the percentage of wound closure were measured. Results （1） All BMDCs expressed CXCR4. （2） The percentages of wound closure at days 7 and 14 in BMDCs group （7 d： 41.3% ±4.6%; 14 d：92.3% ±2. 1%） were significantly higher than those of control group （7 d： 29.3% ±2. 3%; 14 d： 77.3% ±2.5%） and anti-CXCR4 group （7 d： 30.7% ±4.6% ;14 d： 85.7% ±1.5%） （P〈0.05）. The percentage of wound closure of anti-CXCR4 group was significantly higher than that of control group at day 14（P 〈 0.05）. （3） The number of CM-DiI labeled BMDCs at wound site at days 7 and 14 in BMDCs group [7 d： （535 ±84） cells/hpf; 14 d： （769 ±124） cells/hpf） were greater than those of anti-CXCR4 group [7 d： （335 ±97） cells/hpf; 14 d： （521 ± 127） cells/hpf] （P〈0.05）. Conclusions BMDCs participate in the cutaneous wound healing. SDF-1α plays an important role in recruiting BMDCs to wound area.