血管紧张素Ⅱ受体抑制剂对雌激素诱导的Ishikawa细胞的增殖及凋亡的影响

Effect of Angiotensin Ⅱ Receptor Inhibitor on Proliferation and Apoptosis of a Human Endometrial Cancer Cell Line Ishikawa Induced by Estrogen

目的探讨沙拉新(saralasin)对雌激素诱导的ishikawa细胞的增殖、凋亡和细胞周期的影响。方法以不同浓度的药物和时间处理体外培养的ishikawa细胞,采用四甲基亚唑蓝(MTT)法检测ishikawa细胞的生长抑制情况、流式细胞仪碘化丙锭(PI)染色分析细胞周期分布,流式细胞仪及AnnexinⅤ-TITC试剂盒检测saralasin对ishikawa细胞凋亡的影响。结果 saralasin可明显抑制雌激素诱导的ishikawa细胞增殖,使细胞周期停滞在G1/G0期,S期相对减少,凋亡增多。结论 saralasin可以抑制雌激素诱导ishikawa细胞的增殖作用,可能有助于子宫内膜癌的治疗。

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.