兔改良心肌缺血模型的建立

Rabbit Model of Improved Myocardial Ischemia

目的通过改良方法建立简单、有效的兔心肌梗死模型,并与传统方法建立的模型进行心功能和远期存活率的比较。方法健康兔30只,雌雄不拘,随机分为改良模型组(n=15)和传统模型组(n=15)。改良模型组不用气管插管和呼吸机,经胸骨左缘小切口开胸,保持肋骨、胸膜腔完整,在冠状动脉左室支主干第1、2对角支之间闭塞血管1 h后再灌注,建立缺血再灌注心肌梗死模型。传统模型组应用呼吸机,断肋开胸。存活3周后检测心功能并比较存活率。结果 3周后2组心功能均明显下降,左室收缩舒张功能受损;6周时改良模型组存活率高于传统模型组,有统计学差异(P<0.05)。结论使用改良方法可以成功制作兔心肌缺血模型,具有简便、实用、成功率高的特点。

Objective To develop a new rabbit model of myocardial infarction and assess left ventricular function and the survival rates.Methods A total of 30 adult rabbits were equally and randomly assigned into improved（n =15）and traditional model groups（n =15）.The rabbits were not intubated and ventilated in improved model group.A thoracotomy was performed along the left sternum by small nick with rib and pleura cavity integrity.The left anterodecendant arteries（LAD）of adult rabbits were ligated to establish animal models of my-ocardial infarction by a 60-min occlusion of the LAD,followed by reperfusion.In another group,rabbits were intubated and ventilated with rib abscised during thoracotomy inversely.Echocardiographic assessment was performed 3 weeks after myocardial infarction and survival rates were analyzed.Results Echocardiography showed that there was a sizable reduction in systolic and diastolic function in both groups with cardiac function significantly decreased 3 weeks later.Compared with traditional model group,improved method significantly increased the survival rate of rabbit model with myocardial ischemia.Conclusion This improved method is applicable to make an animal model of my-ocardial ischemia in rabbit.We established a simple,rapid,and effective method to mimick myocardial infarction in rabbit.