波尔山羊MyoG基因的鉴定和序列分析

Identification and Sequence Analysis of MyoG Gene in Boer Goat (Capra hircus)

为了探明山羊MyoG基因的序列结构及其对肌肉的分化调控机制,用PCR产物直接测序的方法获得了波尔山羊MyoG基因2363bp长的DNA序列,并对该序列进行了分析。结果表明,波尔山羊MyoG基因包括3个外显子、2个内含子及部分5′UTR(74bp)和3′UTR(260bp)区,编码序列长675bp,共编码224个氨基酸。结构分析表明,该序列所编码的肽链没有信号肽,第1～138个氨基酸为波尔山羊MyoG基因的bHLH结构域。通过比对分析波尔山羊与已知的GenBank中的人类及其它物种MyoG基因发现,该基因编码区核苷酸以及推测的氨基酸同源性和物种间的亲缘关系相一致,无根系统进化树的聚类结果与这些物种本身的生理特性以及传统分类学中已知的生物分类结果相一致。波尔山羊MyoG基因的鉴定及序列分析为山羊肌肉发育调控的机理以及肉质改良等的研究提供了很好的生物学基础信息。

To explore the sequence structure and the regulation mechanism of goat MyoG gene in muscle differentiation,the partial DNA sequences（2 363 bp） were obtained for MyoG gene of Boer goat by direct sequencing,and the sequences were analyzed in other species.The results showed that the sequences of Boer goat MyoG gene consisted of 3 exons,2 introns and some of 5′（74 bp） and 3′（260 bp） untranslated region,with 675 bp coding sequences,encoding a total of 224 amino acids.Structural analysis indicated that the peptide chain had not signal peptide,and a bHLH domain was located at No.1-138 amino acids.Compared Boer goat MyoG with other species,the results showed that the homology of nucleotide of coding region and amino acid accorded with genetic relationship among the species.The cluster result of un-rooted phylogenetic tree was similar to the physiological characteristics of the species and their traditional classification.Identification and sequence analysis of Boer goat MyoG gene provided the important biological information for researching the mechanism of goat′s muscle development and regulation,artificially improving the muscle quality of goat.