2株鸭源H3N2亚型流感病毒的分离鉴定和遗传进化分析

Isolation and Characterization of H3N2 Influenza Viruses from Ducks

2008年10月到2009年9月从扬州活禽市场采集家鸭泄殖腔拭子共1075份,并从中分离鉴定了24株H3亚型禽流感病毒,作者对其中的2株病毒进行了全基因测序和遗传演化分析,发现2株病毒均为H3N2低致病性禽流感病毒。分离病毒各基因遗传进化分析均与猪源分离株的亲缘关系相对较近,与人源分离株的亲缘关系较远;除HA基因外,其它所有的基因均发生了不同程度基因重组,各基因与不同亚型、不同地区、不同宿主分离的不同毒株发生了基因重排。

From October 2008 to September 2009,1 075 cloaca swab samples were collected from ducks in the Live-birds market,and 24 strains of H3 AIVs were isolated.The analysis of complete gene sequences and genetic evolution showed that two H3N2 avian influenza virus were both low pathogenic avian influenza virus and shared high genetic homology with the strains isolated from swine,whereas low to the strains from human.There are various degrees genetic recombination of the genes except HA,among different subtypes in different regions and different hosts,respectively.