年龄相关性白内障晶状体前囊膜中核因子kB的免疫组化研究

Expression of nuclear factor-kappa B in epithelium of human anterior lens capsule with age-related cataract

目的：研究核因子kB蛋白在正常人、年龄相关性白内障的晶状体前囊膜上皮细胞中表达的差异，探讨NF—kB在年龄相关性白内障发病机制中的作用。方法：显微镜下随机收集年龄相关性白内障前囊膜组织30例为病例组及透明晶状体前囊膜组织5例为正常对照组，采用免疫组织化学方法检测NF—kB P65蛋白的表达。结果：正常品状体前囊膜上皮细胞胞质和胞核中可见NF—kB P65蛋白阳性表达，以胞质为主；年龄相关性白内障晶状体前囊膜上皮细胞胞质和胞核染色呈阳性且以胞核为主。图像分析表明病例组NF—kB P65平均吸光度值高于对照组，差异有统计学意义（t=6．2109，P〈0．05）。结论：NF＋kB表达水平升高与年龄相关性白内障发病密切相关，其表达异常可能参与年龄相关性白内障的发生与发展。

AIM： To explore the role of nuclear factor kappa B（ NF- kB） on the occurrence and development of the agerelated cataract by examining the different expression of NF-kB protein Jn epithelium of human anterior lens capsule. METHODS： Thirty anterior capsules with age-related cataract （case group） and 5 normal lens anterior capsules （normal control group） were collected randomly by microscope. Immunohistochemistry was used to detect the level of NF-kB P65 protein and image analysis was adopted to perform the relative quantitative analysis on the two groups. RESULTS： There was NF-kB protein in human lens epithelial cells according to both qualitative and quantitative analysis. Compared wJth normal control group, the expression of NF-kB P65 protein significantly increased in case groups. There was significance based on independent-samples t test （ t = 6. 2109, P〈 0. 05）. CONCLUSION ： NF-kB may be a kind of transcript factor to maintain the elementary metabolic function in lens epithelial cells. The over-expression of NF-kB may play an important role on the occurrence and development of the age-related cataract.