高效液相色谱法测定功劳木中3种生物碱含量

Determination of Three Alkaloids in Chinese Mahonia Stem by HPLC

目的建立测定功劳木中盐酸药根碱、盐酸巴马汀和盐酸小檗碱含量的高效液相色谱(HPLC)法。方法色谱柱为GraceSmartC18柱(250mm×4.6 mm,5μm),以0.05 mol/L磷酸二氢钾溶液(用磷酸调pH至3.0)-乙腈(70∶30)为流动相,检测波长为265 nm,柱温为35℃,流速为1.0 mL/min。结果盐酸药根碱进样量在0.03～1.97μg范围内与峰面积线性关系良好(r=0.999 6),平均回收率为98.45%;盐酸巴马汀进样量在0.04～2.19μg范围内与峰面积线性关系良好(r=0.999 8),平均回收率为100.94%;盐酸小檗碱进样量在0.03～2.12μg范围内与峰面积线性关系良好(r=0.999 9),平均回收率为100.29%。结论该法简便、快速、重现性好,适用于功劳木中盐酸药根碱、盐酸巴马汀和盐酸小檗碱含量的测定。

Objective To establish a HPLC method for the determination of jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem.Methods The analytical column was the GrcaeSmart C18 Column（250 mm × 4.6 mm,5 μm）.The mo-bile phase consisted of 0.05 mol/L potassium dihydrogen phosphate（adjusting pH 3.0 by phosphoric acid）-acetonitrile（70 ∶ 30）.The de-tection wavelength was 265 nm.The column temperature was 35 ℃ and the flow rate was 1.0 mL/min.Results The linearity of jatrorrhizine hydrochloride was in the range of 0.03-1.97 μg,r = 0.999 6,the average sample recovery rate was 98.45%;the linearity of palmatine hydrochloride was in the range of 0.04-2.19 μg,r = 0.999 8,the average sample recovery rate was 100.94%;the linearity of berberine hydrochloride was in the range of 0.03-2.12 μg,r = 0.999 9,the average sample recovery rate was 100.29%.Conclusion This method has good repeatability,flexibility and high sensitivity.It can be used for the determination of jatrorrhizine hydrochloride,palmatine hydrochlo-ride and berberine hydrochloride in Chinese Mahonia Stem.