马钱子抑制兔脊髓损伤的细胞凋亡作用

Nux vomica inhibiting apoptosis of spinal cord injury rabbit

目的:探讨马钱子是否通过抑制细胞凋亡来治疗脊髓损伤。方法:采用改良的Allen's法建立兔脊髓损伤模型。设立假脊髓损伤组、对照组、炙马钱子40mg组(M40组)及炙马钱子80mg组(M80组),分别在给药后1d、7d及用蛋白免疫印迹法(Western blot)检测c-fos蛋白、Bax蛋白和Bcl-2蛋白的表达。结果:与对照组相比,M40组及M80组治疗第7天及14天,可不同程度地抑制损伤脊髓中NO、c-fos蛋白、Bax蛋白的表达,上调Bcl-2蛋白的表达。其中,M40组和M80组分别治疗第7天的NO含量与对照组有统计学差异(P<0.05);M80组治疗第7天时c-fos蛋白与对照组有统计学差异(P<0.05),治疗14d时有重要统计学差异(P<0.01);M80组治疗第7天时Bax蛋白、Bcl-2蛋白及两者的比值与对照组间有统计学差异(P<0.05),M80组治疗14d后Bax蛋白与对照组有统计学差异(P<0.05)。结论:马钱子通过减少损伤脊髓局部NO、c-fos蛋白的表达、下调Bax/Bcl-2比值从而抑制细胞凋亡,阻止脊髓继发性损伤,促进脊髓损伤后神经功能的恢复。

AIM：To explore whether nux vomica treats spinal cord injury by inhibiting apoptosis.METHODS：The animal model of SCI was made in rabbits by modified Allen＇s method.60 rabbits were randomly divided into 4 groups：the sham group,control group,40 mg nux vomica group（group M40）and 80 mg nux vomica group（group M80）.After treating for 1,7 and 14 days,the NO level in the spinal cord was detected with the Griess reaction and the expressions of c-fos protein,tested Bax and Bcl-2 protein were examined by Western blot method（Western blotting）.RESULTS：Compared with the control group,the expressions of NO,c-fos protein,Bax protein were decreased,while the expression of Bcl-2 protein was increased after treating for 7 days and 14 days in the group M40 and group M80.After 7 days＇ treatment,the NO contents in the group M40 and group M80 were lower than the control group（P0.05）.In the group M80,the expressions of c-fos protein were lower than the control group after 7 days＇ treatment（P0.05）and 14 days＇ treatment（P0.01）;the expressions of Bax protein were lower than the control group after 7 days＇ treatment（P0.05）and 14 days＇ treatment（P0.05）;the expression of Bcl-2 protein was higher than the control group after 7 days＇ treatment（P0.05）;the ratio of Bax/Bcl-2 was lower than the control group after 7 days＇ treatment（P0.05）.CONCLUSION：Nux vomica can hold back the secondary spinal cord injury and accelerate the spinal nerve function by reducing the expressions of NO and c-fos protein,and cutting the ratio of Bax/Bcl-2 to suppresse the cell apoptosis.