沼泽红假单胞菌H3对酸性红B_(2GL)染料的厌氧脱色和降解作用

The anaerobic decolorization and degradation of acid red B 2GL by Rhodopseudomonas palustris H3

从印染厂污泥中分离到一株沼泽红假单胞菌（ＲｈｏｄｏｐｓｅｕｄｏｍｏｎａｓｐａｌｕｓｔｒｉｓＨ３），在光照厌氧条件下该菌生长细胞可将１００ｍｇ／Ｌ酸性红Ｂ２ＧＬ染料去除到３０ｍｇ／Ｌ．完整细胞脱色的最适条件为ｐＨ７０，温度３０℃，细胞浓度２０—２５ｍｇ／ｍＬ（湿重）．低浓度的阳离子对脱色影响不大．在通Ａｒ气使严格厌氧和加有还原性辅酶Ｉ的条件下无细胞提取液的脱色活性最高，比活率为１５４×１０－２ｍｇ／（ｍｇ·ｈ）．根据降解产物的分析，推断了该菌对酸性红染料的降解代谢途径．

A strain of Rhodopseudomans palustris with capacity of decolorization was isolated from sludge of printing and dyeing mill. The growing cell could degrade 100mg/L of acid red B 2GL to 30mg/L under anaerobic and illuminated conditions, pH 7.0, temperature 30℃ and cell concentration 20 25mg/mL(wet weight) were optimum conditions for decolorization. The low level metal ions appeared little effects on the decolorization. The addition of NADH and ventilation with argon gas could greatly improve the decolonizing activity of cell free extract, and the highest specific activity was 1.54×10 -2 mg/(mg·h). from chemical analyses of degraded products, a metabolic pathway of acid red B 2GL by bacteria H3 was suggested in this paper.