摘要
目的研究宿主细胞感染马尔尼菲青霉后细胞凋亡、凋亡途径与该菌胞内寄生的相关性。方法马尔尼菲青霉接种于小鼠腹腔内,采用流式细胞术检测4 h、36h的小鼠腹腔细胞凋亡率,用RT-PCR法测定Capase-3、Mcl-1的mRNA水平。结果小鼠腹腔内的炎症细胞凋亡率实验组4h是10.22±3.12,36h是8.22±2.64;空白组4h是23.91±4.10,36h是27.72±8.70;凋亡因子Caspase-3的mRNA的表达量实验组4h是0.65±0.04,36h是0.53±0.02,Mcl-1的mRNA的表达量实验组4h是0.81±0.06,36h是0.92±0.06,与空白组比较其4h、36h均有显著差异性(P<0.05);各组内4h与36h间无明显差异(P>0.05)。结论马尔尼菲青霉可能通过下调Caspase-3 mRNA表达及增加Mcl-1的mRNA表达,从而导致宿主细胞凋亡减少,这可能是马尔尼菲青霉得以存活并随宿主细胞游走播散的致病机制之一。
Aim To investigate the correlation between apoptosis of host cells and the pathogenicity of Penicillium marneffei. Methods Penicillium marneffei were injected intraperitoneally into mice with live yeast in 1 m/of PBS. The apoptosis of the ceils from peritoneal cavity of mice 4 and 36 hours after inoculation was determined with flow cytometry~ The levels of mRNA of Capase3 and Mcl-I expression were detected with RT-PCR technology. Results The quantity of apoptosis ceils in experimental group was less than those of control group 4 hours and 36 hours after inoculation （P〈0.05）. The expression of the apoptosis factor Caspase-3 was lower than the control group 4 hours and 36 hours after experiment（P〈 0.05）. The expression of the inhibitory apoptosis factor Mcl-1 mRNA in experimental group was higher than the control group 4 hours, and 36 hours after test （P〈0.05）. Conclusion Penicillium marneffei induces an antiapoptotic activity on leucocytes by down-regulate the expression of Caspase-3 mRNA and enhance the expression of Mcl-1 mRNA. This might be the survival and pathogenic mechanism for Penicillium marneffei in the host cells.
出处
《中国热带医学》
CAS
2011年第1期16-17,共2页
China Tropical Medicine
基金
广东省自然科学基金资助项目(8151008901000073)
