前突触终末和树突棘之间突触连接的能动性研究

Dynamics of Dendritic Spines and Their Afferent Terminals

目的比较前突触终末和树突棘之间突触连接的能动性。方法运用GFP转染小脑Purkinje细胞、DiI标记平行纤维双标记技术标记样本,双光子显微镜进行活体观察。结果前突触终末与树突棘之间有4种不同的接触方式;树突棘的能动性远大于前突触终末;树突棘和树突棘表面的伪足可主动搜寻附近的前突触终末,与之形成突触连结。结论树突棘的能动性远大于前突触终末,树突棘及其表面的伪足有高度的能动性,提示他们在突触发生过程中发挥特殊作用。

Objective In order to find whether the presynaptic terminals that contact dendritic spines are as dynamic as their postsynaptic targets.Methods Using multiphoton time-lapse microscopy of GFP-labeled Purkinje cells and DiI-labeled granule cell parallel fiber afferents in cerebellar slices.Results There are four kinds of synaptic contacts between dendritic spines and their afferent terminals.Spines are more motile than presynaptic boutons.The continuation of spine motility with functional synaptic boutons in close apposition suggests that spine motility might have roles in synaptic function in addition to facilitating cell-cell contact prior to synaptogenesis.Conclusion Spines are more motile than presynaptic boutons.It suggests that spine motility might have roles in synaptic function that dendritic spines and their filopudium are more motile.