Spondin2在结肠炎发生过程中的表达

Spondin 2 expression is up-regulated during colitis

目的:研究Spondin2的表达调控及其所涉及的信号通路.方法:用含3%葡聚糖硫酸钠饮用水连续喂养C57BL/6小鼠6d,诱导急性结肠炎,然后将小鼠处死,取其结肠组织提取RNA分析.通过定量RT-PCR分析Spondin2 mRNA和核因子-κBp65的表达.应用不同细胞因子和Toll样受体(toll-like receptors,TLRs)配体分别刺激细胞,RT-PCR分析Spondin2 mRNA的表达.结果:与对照组比较,在葡聚糖硫酸钠诱导小鼠结肠炎模型上,Spondin2的mRNA表达上调4.5±0.5倍.TLR-9配体,CpG-ODN刺激野生型RAW264.7细胞后,Spondin2表达上调4.3±0.4倍.结论:在结肠炎发生过程中Spondin2的mRNA表达上调,并可能通过了TLR-9介导的信号通路.

AIM：To investigate the regulation of Spondin 2 expression during colitis and to analyze potential signaling pathways involved.METHODS：C57BL/6 mice were treated with 3% dextran sulfate sodium for 6 d to induce acute colitis.The colon tissue specimens were then harvested for RNA isolation.The mRNA expression of Spondin 2 and nuclear factor-κB p65 was analyzed by quantitative RT-PCR.After RAW 264.7,CMT93 and HEK293 cells were stimulated with different cytokines and Toll-like receptor ligands,the mRNA expression of Spondin 2 was analyzed.RESULTS：The mRNA expression of Spondin 2 was up-regulated by 4.5 ± 0.5 folds in the colon of mice with acute colitis compared with control mice.CpG oligodeoxynucleotide（CpG ODN）,a Toll-like receptor 9（TLR-9） ligand,induced a 4.3 ± 0.4-fold up-regulation of Spondin2 expression in RAW 264.7 cells.CONCLUSION：Spondin 2 expression is upregulated during colitis perhaps in a TLR-9dependent manner.