MIC-1和uPA在食管鳞癌中蛋白表达的相关性及其临床病理意义

Clinical pathological significance of MIC-1 and uPA expression in esophageal squamous cell carcinoma

目的:探讨MIC-1及uPA蛋白表达与食管癌发生、发展及浸润、转移的关系.方法:应用免疫组织化学SP法检测45例食管鳞癌组织、22例癌旁不典型增生组织及45例正常食管黏膜组织中MIC-1及uPA蛋白的表达.结果:食管鳞癌组织中MIC-1蛋白表达与癌的组织学分级、浸润深度、有无淋巴结转移及TNM分期密切相关(P<0.05);uPA蛋白表达与癌的TNM分期及淋巴结转移密切相关(P<0.05);在食管鳞癌癌变过程中MIC-1蛋白表达在癌组织、癌旁不典型增生组织及正常食管黏膜组织中的表达率依次降低,分别为64.4%(29/45)、40.9%(9/22)、13.3%(6/45),组间比较差异具有统计学意义(χ2=24.673,均P<0.01);uPA蛋白在癌组织、癌旁不典型增生组织及正常食管黏膜组织中的表达率也依次降低,分别为68.9%(31/45)、27.3%(6/22)、24.4%(11/45),组间比较差异具有统计学意义(χ2=20.863,P<0.01);MIC-1与uPA的表达呈正相关关系(γp=0.403,均P<0.01).结论:MIC-1和uPA在食管癌的浸润、转移及黏膜上皮癌变过程中起重要作用,MIC-1及uPA的联合检测可望成为食管鳞癌早期诊断和判断预后的分子指标之一.

AIM： To explore the relationship of the protein expression of macrophage inhibitory cytokine-1 （MIC-1） and urokinase plasminogen activator （uPA） with the development, progression, invasion and metastasis of esophageal squamous cell carcinoma （ESCC）. METHODS： The protein expression of MIC-1 and uPA was detected by immunohistochemistry using the streptavidin-peroxidase method in 45 ESCC specimens, 22 tumor-adjacent atypical hyperplastic epithelial specimens and 45 normal esophageal epithelial specimens.RESULTS： The expression of MIC-1 protein was closely correlated with tumor grade, infiltration, TNM grade and lymphatic metastasis in ESCC （all P 0.05）. The expression of uPA protein was closely correlated with tumor TNM grade and lymphatic metastasis in ESCC （both P 0.05）. The positive rates of MIC-1 expression in ESCC, tumor-adjacent atypical hyperplastic epithelium and normal esophageal epithelium were 64.4% （29/45）, 40.9% （9/22） and 13.3% （6/45）, respectively, with a significant difference among the three groups （χ2 = 24.673, P 0.01）. The positive rates of UPA protein expression in ESCC, tumor-adjacent atypical hyperplastic epithelium and normal esophageal epithelium were 68.9% （31/45）, 27.3% （6/22） and 24.4% （11/45）, respectively, with a significant difference among the three groups （P 0.01）. There is a positive correlation between the protein expression of MIC-1 and uPA （γp = 0.403, P 0.01）. CONCLUSION： MIC-1 and uPA play important roles in the carcinogenesis, infiltration and metastasis of ESCC. Combined detection of MIC-1 and uPA expression may be a promising molecular parameter for early diagnosis and prognostic evaluation of ESCC.