谷胱甘肽转硫酶基因多态性与浙江汉族溃疡性结肠炎易感性的相关性

Relationship between genetic polymorphisms of glutathione S-transferase genes and susceptibility to ulcerative colitis in Zhejiang Han population

目的:探讨谷胱甘肽转硫酶(GSTs)基因多态性与浙江汉族溃疡性结肠炎(UC)易感性的关系.方法:选取浙江省温州医学院附属第二医院和温州市其他大型综合性医院UC患者252例,对照组共578例,系同期温州医学院附属第二医院体检中心的健康体检者.采用聚合酶链反应技术检测GST(M1、T1、P1)基因多态性在UC患者和正常对照者之间的分布差异.结果:UC患者中GSTM1(-)、GSTT1(-)和GSTP1(VaL/VaL)基因型频率明显高于正常对照组(69.45%vs46.30%,P=0.0003;61.51%vs51.39%,P=0.007;48.81%vs34.61%,P<0.0001).进一步根据UC临床症状分层分析,在远端结肠炎患者中GSTT1(-)、GSTP1(VaL/VaL)基因型的分布频率高于广泛性结肠炎患者(P=0.0001,P=0.001);而GSTM1(-)基因型与UC的病变部位无相关性(P=0.108).并且GST(M1、T1、P1)突变基因型与UC患者病情严重程度无关(均P>0.05).结论:GST(M1、T1、P1)基因突变与浙江汉族UC明显相关.

AIM： To investigate the association between the genetic polymorphisms of glutathione S-transferases （GSTs） genes and susceptibility to ulcerative colitis （UC） in Zhejiang Han population. METHODS： A total of 252 patients with UC were collected from the Second Affiliated Hospital of Wenzhou Medical College and other large general hospitals in Wenzhou City. The control group was composed of 578 healthy volunteers. PCR was used to examine the prevalence of GST （M1, T1, P1） gene polymor-phisms in these subjects. RESULTS： The frequencies of GSTM1 （-）, GSTT1 （-） and GSTP1 （VaL/VaL） were significantly higher in UC patients than in controls （69.45% vs 46.30%, P = 0.0003; 61.51% vs 51.39%, P = 0.007; 48.81% vs 34.61%, P 0.0001）. A further observation was made on the UC patients according to the clinical features. The frequencies of GSTT1 （-） and GSTP1 （VaL/VaL） genotypes were higher in patients with distal colitis than in those with extensive colitis （P = 0.0001 and 0.001）. However, the same result was not observed for GSTM1 （-） genotype （P = 0.108）. In addition, the variant genotypes of GST （M1, T1, P1） were not significantly linked to the severity of the disease （all P 0.05）. CONCLUSION： The GST （M1, T1, P1） variant genotypes are obviously correlated with the development of UC in Zhejiang Han population.