TKTL1蛋白及mRNA在食管鳞癌组织中的表达及其临床意义

Clinical significance of TKTL1 expression in esophageal squamous cell carcinoma

目的:探讨TKTL1在食管鳞状细胞癌中的表达及其临床意义.方法:分别采用原位杂交及免疫组织化学的方法检测62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中TKTL1蛋白及其mRNA的表达.结果:正常黏膜组织、癌旁不典型增生组织及食管鳞癌组织中TKTL1蛋白阳性表达率依次增高,分别为17.7%(11/62)、29.0(9/31)、53.2%(33/62),组间比较差异有统计学意义(均P<0.05);TKTL1蛋白表达与癌的浸润深度、淋巴结转移及TNM分期密切相关,组间比较差异有统计学意义(均P<0.05).正常黏膜组织、癌旁不典型增生组织及食管鳞癌组织中TKTL1mRNA的阳性表达率依次增高,分别为4.8%(3/62)、22.6%(7/31)、43.5%(27/62),组间比较差异有统计学意义(均P<0.05);TKTL1mRNA表达与癌的浸润深度、淋巴结转移及TNM分期密切相关,组间比较差异有统计学意义(均P<0.05).TKTL1蛋白及其mRNA的表达呈正相关关系(γp=0.302,P<0.01).结论:TKTL1蛋白及其mRNA表达率升高可能与食管鳞癌的发生、发展及浸润、转移有关.

AIM： To investigate the expression of transketolase like-1 （TKTL1） protein and mRNA and to analyze its clinical significance in esophageal squamous cell carcinoma （ESCC）. METHODS： The expression of TKTL1 protein and mRNA was detected by immunohistochemistry and in situ hybridization in 62 ESCC specimens, 31 tumor-adjacent atypical hyperplastic epithelial specimens, and 62 normal esophageal mucosal specimens, respectively.RESULTS： The positive rates of TKTL1 protein expression in normal esophageal epithelium, tumor-adjacent atypical hyperplastic epithelium and ESCC were 17.7% （11/62）, 29.0% （9/31） and 53.2% （33/62）, respectively, with significant differences among the three groups （all P 0.05）. TKTL1 protein expression was closely correlated with infiltrative depth, lymphatic metastasis and TNM grade in ESCC （all P 0.05）. The positive rates of TKTL1 mRNA expression in normal esophageal epithelium, tumor-adjacent atypical hyperplastic epithelium and ESCC were 4.8% （3/62）, 22.6% （7/31） and 43.5% （27/62）, respectively, with significant difference among the three groups （all P 0.05）. TKTL1 mRNA expression was closely associated with infiltrative depth, lymphatic metastasis and TNM grade in ESCC （all P 0.05）. There is a positive correlation between TKTL1 mRNA and protein expression （γp = 0.302, P 0.01）. CONCLUSION： TKTL1 expression may be associated with the development, invasion and metastasis of ESSC.