Gallic acid和SDS对黑麦草体内重金属亚细胞及形态分布的影响

Influence of gallic acid and SDS on the subcellular and chemical form distribution of heavy metals in Ryegrass ( Lolium perenne L. )

通过盆栽试验,研究了投加螯合剂没食子酸gallic acid(5mmol·kg-1)与表面活性剂十二烷基磺酸钠SDS(1mmol·kg-1)对黑麦草(Lolium perenneL.)体内重金属亚细胞及形态分布的影响.结果表明,单独投加gallicacid增强了黑麦草对Cd、Pb的区室化作用及对Zn的细胞壁沉积作用;复合投加gallic acid和SDS增强了黑麦草对Cd的区室化作用和细胞壁沉积作用,以及对Zn的细胞壁沉积作用.单独投加gallic acid使黑麦草体内Cd、Zn总量分别增加了33.5%和6.0%,其中,惰性态Cd、Zn所占比例分别增加了6.2%、6.3%;复合投加gallic acid和SDS使黑麦草体内Cd总量增加了35.6%,Zn总量减少了1.1%,其中,惰性态Cd、Zn所占比例分别增加了7.2%、8.6%,增强了植物对Cd、Zn的螯合作用.各处理对Pb的亚细胞分布及形态无明显影响.

The commonly used chelating agent gallic acid （3,4,5-trihydroxybenzoic acid,5 mmol·kg^-1） ,the surfactant SDS （sodium dodecyl sulfate, 1 mmol·kg^-1） and the accumulator Lolium perenne L. were selected for use in pot experiments to investigate the effects of gallic acid and SDS on the subcellular distribution and chemical form of heavy metals in Lolium perenne L. The results showed that dosing with gallic acid enhanced the role of Lolium perenne L. in compartmentalizing cadmium and lead,and depositing zinc. Dosing with both gallic acid and SDS enhanced the compartmentalizing and depositing of cadmium,and depositing of zinc. Adding gallic acid alone increased the uptake of total cadmium by 33. 5% and zinc by 6. 0% ,while the non-labile cadmium and zinc were increased by 6. 2% and 6. 3% ,respectively. By dosing with gallic acid and SDS,the total cadmium increased by 35. 6% but zinc dropped by 1. 1% ; the non-labile cadmium and zinc were increased by 7. 2% and 8. 6% ,respectively,and the chelation of cadmium and zinc was enhanced. The subcellular distribution and chemical form of lead did not change under either set of conditions.