佛肚竹竹叶提取物的抗菌作用

Study on antimicrobial effect of the extracts from Bambusa vulgaris cv. Wamin leaf

通过正交实验得出佛肚竹竹叶最佳抑菌提取液的提取条件为:料液比1∶15,乙醇浓度60%,提取温度70℃;提取液对大肠杆菌的抑制作用优于其对枯草杆菌和金黄色葡萄球菌;竹叶冷冻干燥后所得的提取液的抑菌效果优于自然风干法;对枯草杆菌的最小抑菌浓度为0.125%,对金黄色葡萄球菌的为0.25%,对大肠杆菌的为0.125%。Vc对竹叶提取液具有协同抑菌作用;竹叶提取物与细菌作用时间越长,其抑菌效果越好。

The results of orthogonal experiments showed that the extracted conditions of Bambusa vulgaris cv. Wamin leaf which had best antimicrobial effect were： solid -liquid ratio 1 ∶15, concentration of alcohol 60% , leaching-temperature 70 ℃. The antimicrobial effects of the extracts had been detected by inhibition zone method. The results showed that the inhibition of the extracts again E.coli. was stronger than again B.subtilis and S.aureus. The extracts from the leaf by vacuum freezing-dry has stronger antimicrobial effect than that by air-dry. The minimal inhibitory concentrations（MIC） were： B.subtilis 0.125%, S S.aureus. 0.25%, E.coli. 0.125%. The extraction with Vc can offer synergistic bacteriostatic affect. The longer the bacteria and the extraction fixed with each other, the stronger the antimicrobial effect was.