我国不同产地绿豆抗氧化活性分析

Analysis of antioxidant capacity of mung beans from different areas in China

目的：测定我国十四个产地的绿豆样品的总酚含量、总黄酮含量以及抗氧化能力，为我国部分绿豆品种品质评价体系提供科学依据。方法：分别采用福林-酚法、三氯化铝显色法测定绿豆样品的总酚含量、总黄酮含量，采用铁离子还原抗氧化法（FRAP值）、N，N-二苯基三硝基苯肼（DPPH）自由基清除率法评价其抗氧化能力。结果：绿豆样品总酚含量为2．85-3．84mg没食子酸／g，总黄酮含量为1．99～2．53mg儿茶素／g，FRAP值为1．39～2．08mmol Fe^2＋／100g，DPPH为1．40-2．03mg抗坏血酸／g。样品总酚含量与FRAP值（r=0．84，P〈0．01）、总黄酮与DPPH（r=0．89，P〈0．01）相关关系显著。结论：不同产地绿豆总酚含量、总黄酮含量、FRAP值、DPPH存在显著差异。绿豆的抗氧化活性与其总酚含量、总黄酮含量显著正相关，与淀粉、蛋白质、粗纤维、灰分、脂肪含量没有显著的相关性．

Objective：In order to provide scientific evidence for quality evaluation system based on some mung bean cultivars in China, the total phenolic content （TPC）, total flavonoid content （TFC） and the antioxidant capacity of mung beans from fourteen different areas in China were determined.Methods：The TPC was determined by a Folin -Ciocalteu assay.The TFC was determined by an aluminum chloride chromotest.Ferric reducing antioxidant power （FRAP value） ,2,2- diphenyl- 1 - picrydrazyl radical scavenging assay （ DPPH value） was used for analyzing antioxidant properties.Results：The TPC of mung bean samples ranged from 2.85 to 3.84mg gallic acid/g,the TFC ranged from 1.99 to 2.53mg （ ＋ ）-catechin/g, FRAP values ranged from 1.39 to 2.08mmol Fe^2＋/100g, DPPH values ranged from 1.40 to 2.03mg ascorbic acid/g.There were significant linear correlations between TPC and FRAP values（ r =0.84, p 〈 0.01 ）, TFC and DPPH values （ r = 0.89, p 〈 0.01 ）. Conclusion： TPC, TFC, FRAP values, DPPH values of the mung bean samples from different areas were significantly different. Antioxidant activities were strongly correlated with TPC, TFC. But no significant correlations exsited between starch, protein, crude fiber, ash content,fat and antioxidant activities.