摘要
利用本实验室分离保存的乳酸菌菌种17株,经过耐胃液实验筛选,得到耐胃液能力较强、生长良好的乳酸菌菌株5株,选取4株菌株利用API乳酸菌生化系统和16S rDNA分析鉴定,并进行耐胃液驯化选育。结果表明,鉴定的4株乳酸菌中2株(编号:BR1-2和R8)为副干酪乳杆菌(Lactobacillus paracasei),1株(编号:BR3-2)为屎肠球菌(Enterococcus faecium),1株(编号:BR2-2)为乳酸片球菌(Pediococcus acidilactici);驯化后各菌株耐胃液能力均有不同程度的提高,其中BR1-2经人工胃液处理存活率提高52.2%;其他三株菌株的存活率也均提高20%以上。
By gastric resistant test,5 gastric tolerable strains were screened from 17 strains of the conserved strains.With API lactic acid bacteria biochemistry system and 16S rDNA analysis,the gastric tolerable property of the 4 strains of the 5 strains were domesticated.The results showed that 2 strains of the 4 identified strains（BR1-2 and RS） were Lactobacillus paracaseL Another 2 strains, one was Enterococcus faecium, the last one was Pediococcus acidilactici.After the domestication,the gastric resistant ability of the 4 strains was higher than before. The survival rate of BR1-2 was advanced by 52.2% ,the other 3 strains＇ survival rates were advanced over 20%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第11期190-192,195,共4页
Science and Technology of Food Industry
基金
粤港关键领域重点突破项目招标项目(2007168603)
关键词
人工胃液
驯化
乳酸菌
耐胃液能力
artificial gastric
domestication
lactic acid bacteria
gastric resistance
