一种新型的分子内电荷转移荧光探针测定人血清白蛋白

Determination of Human Serum Albumin Using a New Intramolecular Charge Transfer Fluorescence Probe

由于人血清白蛋白（HSA）与一种分子内电荷转移（ICT）化合物1-酮-2-（对二甲氨基苯亚甲基）-四氢萘（KDTN）的结合反应,引起KDTN荧光的增强,其荧光强度的增强与HSA的浓度在13.6~272.0 mg.L-1范围内呈线性关系。据此提出了以KDTN为探针测定人血清白蛋白的荧光方法。方法的检出限（3S/N）为0.136 mg.L-1,用标准加入法对方法的回收率作了试验,测得平均回收率为104.4%,测定值的相对标准偏差（n=7）为4.1%。

Due to the combination reaction between human serum albumin（HSA） and the intromolecular charge transfer（ICT） compound,1-keto-2-（p-dimethylaminobenzal）-tetrahydronaphthalene（KDTN）,fluorescence of which was found to be enhanced.Linear relationship between the magnitude of the enhanced fluorescence intensity and mass concentration of HSA was found in the range of 13.6-272.0 mg·L-1.Based on these facts,the new ICT fluorescence probe was proposed to be used for determination of HSA.Detection limit（3S/N） of this method was found to be 0.136 mg·L-1.Recovery and precision of the method were tested by standard addition method,giving values of average recovery of 104.4% and RSD′s（n=7） of 4.1%.