酶谱法测定肝星状细胞合成的明胶酶活性

Zymography identification of the gelatinase synthesized by cultured hepatic stellate cells

目的建立检测肝星状细胞(HSC)合成的明胶酶(MMP-2,MMP-9)活性的方法。方法用含SDS-明胶的聚丙烯酰胺凝胶电泳为基础的酶谱法,检测体外培养的HSC所合成的明胶酶活性。结果酶谱法能灵敏地检测到HSC分泌到培养基中的MMP-2和MMP-9,培养基中MMP-2活性高于MMP-9,并观察到药物作用以后酶活性的改变。结论酶谱法是研究HSC调节肝纤维化过程中细胞外基质(ECM)代谢的有效手段。

Objective： To establish a method for identification of the activities of gelatinase（MMP-2,MMP-9）synthesized by cultured hepatic stellate cells（HSC）.Methods：SDS-gelatin polyacrylamide gel electrophoresis was used for the identification of gelatinase synthesized by cultured HSC.Results：Gelatinase in culture medium was sensitively identified,and MMP-2 activity was higher than MMP-9 in culture medium,and the changes of gelatinase in culture medium were also observed after 24h medicine treatment.Conclusion：Zymography is suitable for the studies on the role of HSC in the regulation of extracellular matrix turnover.