基线等比增减设计法优选PNS/TGCO组分对实验性气虚血瘀型冠心病的影响研究

Optimization of PNS/TGCO Components Effect on Experimental Qi Stagnation and Blood Stasis Type of Coronary Heart Disease with Increase-decrease Baseline Geometric Proportion Design Method

目的:初步探索三七总皂苷(PNS)和山茱萸总苷(TGCO)配伍治疗气虚血瘀型冠心病的作用及特点。方法:采用基线等比增减设计法拟定PNS/TGCO组合配比,观察不同比例组合的有效组分对垂体后叶素所致大鼠急性心肌缺血的影响以及对正常小鼠耐缺氧时间的影响。结果:处方1、2、3及血塞通有一定改善大鼠心肌缺血程度的作用,处方4、5、6与对照组比较无显著性差异;处方3、4、5、6有一定抗小鼠缺氧的作用,处方1、2及血塞通组与对照组比较无显著性差异。结论:处方3既有一定改善大鼠心肌缺血的作用又有一定抗小鼠缺氧的作用,为PNS/TGCO治疗气虚血瘀型冠心病有效组分的最佳配比。

Objective：Preliminary exploration of Panax notoginseng saponins（PNS）and total saponins from Cornus officinalis（TGCO）components treatment and characteristics of Qi stagnation and blood stasis type of coronary heart disease.Methods：With increase-decrease baseline geometric proportion design method to Optimization of PNS/TGCO components,through observing different proportions effective components effect on mice with pituitrin-induced acute myocardial ischemia and the time of hypoxia tolerance.Results：Prescriptions 1,2,3 and Xuesaitong groups had a certain degree of improvement the role of myocardial ischemia and prescriptions 4,5,6,compared with the control group with no significant changes;Prescriptions 3,4,5,6 had a certain role in anti-anoxia in mice,prescriptions 1,2 and Xuesaitong group,together with the control group with no significant changes.Conclusion：Prescription 3 has some effect on improving the role of myocardial ischemia and has a certain role in anti-anoxia.It′s the best ratio of PNS / TGCO components for the treatment of Qi stagnation and blood stasis type of coronary heart disease.