刺芒柄花素雌激素样作用及对大鼠心房雌激素受体表达的影响

The Estrogenic Effect of Formononetin and Its Effect on the Expression of Rats′ Atrium Estrogen Receptors

目的:探讨刺芒柄花素是否具有雌激素样作用及对大鼠心房雌激素受体(ERα和ERβ)表达的影响。方法:健康成年SD雌性大鼠50只,随机分为假手术组、模型组、尼尔雌醇组及刺芒柄花素低、高剂量组。后4组行双侧卵巢切除术,术后第5～10 d每天1次进行阴道冲洗脱落细胞涂片。去势成功后,假手术组和模型组灌胃生理盐水10 mL/(kg.d),尼尔雌醇组灌胃尼尔雌醇2.5 mg/(kg.w),刺芒柄花素低、高剂量组分别灌胃刺芒柄花素20、100 mg/(kg.d),连续8 w。第8周后连续5 d作阴道冲洗细胞涂片,第8周末断头处死大鼠,取出子宫称量质量后染色镜检。取右心房组织行RT-PCR法测定ERα、ERβ的相对表达量。结果:连续用药8 w后,高剂量刺芒柄花素能促使大鼠阴道上皮产生性周期变化并促进去势后萎缩的子宫质量增加。高剂量刺芒柄花素能上调ERβ表达(P<0.01),而对ERα的表达无明显影响(P>0.05)。结论:刺芒柄花素具有雌激素样作用,能选择性提高大鼠心房ERβ表达。

Objective：To observe the estrogenic effect of formononetin and its effect on the expressions of atrial estrogen receptor subtypes alpha and beta（ERα and ERβ.）.Methods：50 femal rats were randomly divided into five groups：sham group,model group,nilestriol group,formononetin groups of low and high dose.Rats in sham group were cut a piece of fat before closing the abdomen,the others were ovariectomized.Vaginal exfoliated cell were observed from the fifth day to the tenth after operation to test if the moddle is successful.The sham and model group were given nomal saline in 10 mL/kg by gavage,the remaining three groups were given nilestriol 2.5 mg/（kg·w）,low[20 mg/（kg·d）]and high dose[100 mg/（kg·d））of formononetin by gavage respectively.In the 8th week,vaginal exfoliated cell were observed,then decapitated the rats,removed the uterus,weighed and take wright staining microscopy.The relative expressions of ERα and ERβ of right atrium were detected by RT-PCR.Results：The vaginal cells exhibit a change of estrus after had been fed with high dose of formononetin after 8 weeks.Formononetin increase the uterus coefficient and the expression of atrial ER β（P0.01）,but it dose not have any effect on the expression of ERα（P0.05）.Conclusion：Formononetin have estrogenic effect in ovariectomized rats,and it can markedly upregulate the expression of rats′ atrial ER β.