减压内沸法提取湿地松松针中莽草酸的研究

Study on Extraction of Shikimic Acid from Pine Needles of Pinus elliottii by Decompressing Inner Ebullition

以湿地松松针为原料,高浓度乙醇为解吸剂,低浓度乙醇为热提剂,采用减压内部沸腾提取法,对湿地松松针中莽草酸进行提取工艺的研究。通过单因素实验和正交试验,确定提取最佳工艺是:当乙醇浓度70%,解吸时间18 min,热提温度65℃,热提时间4 min,解吸时料液比1∶1.6,热提剂用量260 mL,热提剂浓度30%,提取2次时,提取效果最好,湿地松松针干基中莽草酸的提取率为1.51%。

We studied the extracting process of shikimic acid from pine needles of Pinus elliottii Engelm,using pine needles of Pinus elliottii Engelm as raw materials,a high concentration of alcohol as the desorption and a low concentration of alcohol as the hot-extraction,and the decompressing inner ebullition method.Through the single factor test and orthogonal test,the best processing of the decompressing inner ebullition was as follows：when the concentration of alcohol was 70 percent with 18 minutes′ desorption,the temperature of hot-extraction was 65 ℃ with 4 minutes′ hot-extraction,solid-liquid ratio was 1∶1.6 with 260 mL of hot-extraction,with a concentration of 30%.The extracting effect was the best when extracted twice.The extracting proportion of shikimic acid from dry pine needles of Pinus elliottii was 1.51%.