洛伐他汀对RAW264.7体外生长抑制作用的研究

Study of lovastatin on inhibition of tumor cell

目的通过细胞培养,观察洛伐他汀(lovastatin,LOV)对小鼠巨噬细胞株RAW264.7的作用,探讨LOV对小鼠巨噬细胞株(RAW264.7)增殖和凋亡的影响。方法以不同浓度(20,40,80μmol/L)LOV处理RAW264.7,作用不同时间(24 h,48 h,72 h),以MTT法检测LOV对小鼠巨噬细胞RAW264.7增殖的影响;观察洛伐他汀对小鼠巨噬细胞RAW264.7凋亡的影响。结果洛伐他汀对小鼠巨噬细胞瘤RAW264.7有明显的抑制生长的作用(P<0.05),细胞凋亡形态观察显示,经80μmol/L洛伐他汀作用的细胞出现明显固缩的凋亡小体。结论洛伐他汀能有效抑制巨噬细胞株的体外生长,其作用与剂量、时间具有一定的依赖性,这种抑制作用可能与洛伐他汀诱导细胞凋亡有关。

Objective： To explore the effect of lovastatin（LOV） on proliferation and apoptosis of RAW264.7.Methods： After the treatment of RAW264.7 with various concentration LOV（20μmol/L,40μmol/L,80μmol/L）for 24,48,and 72 hours,the proliferation was oberseved by MTT,the effect of LOV on apoptosis of RAW264.7 was observed from fluorescence microscopy.Results： LOV could inhibit the proliferation of cells effectively（P〈0.05） and apoptotic body could be observed from fluorescence microscopy.Conclusion： LOV can inhibit the proliferation of cancer cells effectively.All the effects above are dependent on dose and time.The inhibition effect may be related to LOV in inducing the cell apoptosis