新疆部分维吾尔族HIV-1感染和高危人群RANTES SDF1-3′A基因的多态性分析

Analysis of polymorphism of RANTES and SDF1-3'A genes related to HIV-1 infected population and high-risk population in a group of Urghur ethnic in Xinjiang

目的分析新疆维吾尔族275例艾滋病病毒Ⅰ型(HIV-1)感染者和275名高危人群,对激活T细胞表达和分泌调节(RANTES)和基质细胞衍生因子(SDF1)-3′A等位基因的多态性和分布特点。方法利用聚合酶链反应-限制性内切酶片段长度多态性分析(PCR-RFLP)法,对维吾尔族高危人群和HIV-1感染者的各275份血液中的RANTES、SDF1-3′A等位基因进行检测。结果在275例高危人群中,RANTES、SDF1-3′A等位基因突变率分别为14.2%、19.7%,在275例HIV-1感染人群中,RANTES、SDF1-3′A等位基因突变率分别为19.6%、24.7%。χ2检验的结果提示,维吾尔族高危人群和HIV-1感染人群中,RANTES、SDF1-3′A基因多态性分布差异无统计学意义(P>0.05)。结论 RANTES、SDF1-3′A等位基因突变以及分布提示,在新疆维吾尔族人群HIV-1高危人群和感染人群中无差异性。

Objective To analyze the frequency and polymorphism of the allele of RANTES and SDF1-3＇A genes related to 275 high-risk cases and 275 HIV-1 infected cases among Urghur ethnic in Xinjiang.Method The allele of RANTES and SDF1-3′A genes from 275 blood samples in high-risk cases and infected cases were detected respectively by using PCR-RFLP method.Results In 275 high-risk cases,the mutation rate of allele of RANTES and SDF1-3′A genes were 14.2% and 19.7% respectively,and 19.6% and 24.7% of mutation rate were obtained in 275 infected cases（P〉0.05）.Conclusion The mutation and frequency of the allele of RANTES and SDF1-3′A genes show no difference between HIV-1 infected population and high-risk population.