高效液相色谱法测定加味青娥丸中补骨脂素、异补骨脂素的含量

Determination of Psoralen and Isopsoralen in Jiawei Qing'e Pills by HPLC

目的:测定加味青娥丸中补骨脂素、异补骨脂素的含量。方法:色谱柱:Zorbax C_(18)(4.6mm×150mm,5μm),流动相:乙腈-水(30:70),流速:1.0mL·min^(-1),检测波长:246nm。结果:补骨脂素、异补骨脂素分别在0.0258～0.2580μg、0.0240～0.2400μg范围内线性良好(r=0.9999,n=6),回收率分别为100.56%、100.74%,RSD分别为0.75%和1.00%(n=6)。结论:该方法简便、快速、灵敏、准确,可作为加味青娥丸中补骨脂的含量测定方法。

This work aimed to establish a method for HPLC determination of psoralen and isopsoralen in Jiawei Qing-e Pills. The condition for HPLC was the Zorbax C18 column （150 mm×4.6 mm, 5μm, Agilent）. The detection wavelength was 246 nm. The mobile phase consisted of acetonitrile-water （30：70）. For psoralen, the linear range of psoralen was determined to be 0.0258-0.2580 μg, r=0.9999 （n=6） with an average recovery rate of 100.56%（RSD= 0.75%）; for isopsoralen, the liner range was 0.0240-0.2400 μg,r=0.9999 （n=6） with an average recovery rate of 100.74%（RSD=1.00%）. This method shows good separating results, accuracy and reproducibility, thus it may be used for the quality control of Jiawei Qing-e Pills.