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鸡卵清蛋白水解物中分离纯化出具有清除DPPH·自由基的多肽的研究 被引量：1

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摘要 本文利用胰凝乳蛋白酶水解鸡卵清蛋白,得到鸡卵清蛋白水解物。通过包括凝胶过滤和反相高效液相法等连续柱层析法分离纯化得到一种具有较强DPPH·自由基清除能力的多肽。液质联用鉴定该物质的相对分子质量为730.4Da,结构为Cys-Pro-Asp-Gly-Asn-Ile-Leu,位于卵粘蛋白α亚单元的1742～1748,其DPPH·自由基清除率为27%(500μg/mL)。
作者 成静 于兰 杨国燕 唐良艳 季金林 陈栋梁
机构地区 武汉肽类物质研究所 武汉好普生物制品检测有限公司 中华医学会健康管理学分会抗衰老医学实验室
出处 《中国食品工业》 2010年第9期62-65,共4页 China Food Industry
关键词 鸡卵清蛋白肽 DPPH·清除自由基能力 质谱仪
分类号 S879.3 [农业科学—畜牧兽医]
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参考文献21

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同被引文献14

  • 1徐力,李相鲁,吴晓霞,王华,侯瑞珍,黄宜兵,张学忠.一种新的玉米抗氧化肽的制备与结构表征[J].高等学校化学学报,2004,25(3):466-469. 被引量：63
  • 2GB/T5009.5-2010食品中蛋白质的测定[S].北京:中国标准出版社,2010.
  • 3F奥斯伯 R布伦特 RE金斯顿 等.精编分子生物学实验指南[M].北京：科学出版社,2001.305.
  • 4E.Mendis, N.Rajapakse.Antioxidant properties of a radical-scav- enging peptide purified from enzymatically prepared fish skin gela- tin hydrolysate[J].Journal of Agricultural and Food Chemistry, 2005,53(3) :581-587.
  • 5Elizabeth Escudero, Leticia Mora.Identification of novel antioxi- dant peptides generated in Spanish dry-cured ham[J].Food Chemis- try,2013,138(2-3) : 1282-1288.
  • 6GB/T22729-2008海洋低聚肽粉[s].北京:中国标准出版社,2008.
  • 7GB/T5009.39-2003酱油卫生标准的分析方法[S].北京:中国标准出版社,2004.
  • 8Chen H.M, Koji Muramoto.Structural analysis of antioxidative pep- tides from soybean I-conglycinin[J].Journal of Agricultural and Food Chemistry, 1995,43 (3) : 574-578.
  • 9N.Rajapakse, E.Purification and in vitro antioxidative effects of gi- ant squid muscle peptides on free radical-mediated oxidative sys- tems[J].The Journal of Nutritional Bioehemistry, 2005,16(9) :562- 569.
  • 10赵晓丽,陈智毅,刘学铭.菌糠的高效利用研究进展[J].中国食用菌,2012,31(2):1-3. 被引量：80

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中国食品工业
2010年 第9期

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