硫酸化多糖复方对鸡脾脏和外周血淋巴细胞增殖的影响

Effects of Sulfated Polysaccharide Prescriptions on Chicken Splenic and Peripheral Lymphocyte Proliferation

筛选出具有增强鸡淋巴细胞增殖作用的硫酸化多糖复方。以前期筛选出的硫酸化黄芪多糖sAPS60、硫酸化淫羊藿多糖sEPS5和硫酸化香菇多糖sLNT2组成4个复方sPSP1、sPSP2、sPSP3和sPSP4,测定它们对鸡脾淋巴细胞的安全浓度后,取安全浓度范围内的各复方分别单独或与植物血凝素(PHA)、细菌脂多糖(LPS)或伴刀豆素球蛋白(ConA)加入到培养的鸡脾脏或外周血淋巴细胞的培养体系中,培养48 h后,用四甲基偶氮唑蓝(MTT)法测定淋巴细胞增殖的变化。4种硫酸化多糖复方可以单独或协同PHA、LPS显著刺激鸡脾淋巴细胞增殖、协同ConA显著刺激外周血淋巴细胞,且有一定的浓度依赖关系,综合评价以sPSP1、sPSP2的作用最强。

To select the effective sulfated polysaccharide prescriptions（sPSPs） on enhancing the lymphocyte proliferations,four sPSPs（sPSP1,sPSP2,sPSP3 and sPSP4） were prepared with sulfated astragalus polysaccharides（sAPS60）,sulfated epimedium polysaccharide（sEPS5） and sulfated lentinan（sLNT2） selected in previous experiment.After the safe concentrations were tested,the four sPSPs were added into the cultivation system of chicken splenic or peripheral lymphocyte,singly or synergistically with PHA,LPS or ConA.After cultivated for 48 h,the lymphocyte proliferations were determined by MTT method.The results showed that the four sPSPs could significantly stimulate the splenic lymphocyte proliferation singly or synergistically with PHA and LPS,and stimulate peripheral lymphocyte proliferation synergistically with ConA.There were definite dose-effect relationships beween the concentrations of sPSPs and the stimulation effects.In comprehensive evaluation,sPSP1 and sPSP2 possessed the best efficacy.