黑海参酸性粘多糖的分离纯化

Extraction and Purification of Acidic Mucopolysaccharide from Holothuria atra

采用酶法和碱法消化、醋酸钾和乙醇分级沉淀,从新鲜黑海参体壁中分离得到酸性粘多糖,分子量为10253Da,经琼脂糖电泳证实为纯一成分,多糖组成中氨基半乳糖、葡萄糖醛酸、岩藻糖、硫酸基含量分别为16.12％、17.88％、11.66％、23.52％。

Mucopolyaccharide with molecular weight of 10253 Da was extracted and purified from fresh Holothuria atra Jaeger by means of enzymic and alkaline hydrolysis, potassium acetate and ethanol fractional precipitation.It was tested to be purified ingredient with agarose electrophoresis.The percentage content of galactosamine, glucuronic acid, fucose and sulfate in the mucopolysaccharide was 16.12% , 17.88% , 11.66%and 23.52% respectively.