SiO_2刺激THP-1上清液引起早期矽结节样病变

Early Silicotic Noge like Lesion Induced by SiO 2 Stimulated THP 1 Supernatants

目的：探索ＳｉＯ２刺激ＴＨＰ－１细胞（具有肺泡巨噬细胞特性的人血单核细胞株）上清液是否可引起大鼠的矽肺样病变。方法：用ＳｉＯ２刺激ＴＨＰ－１细胞培养上清液经非暴露式气管内注入，０．５ｍｌ／次，１次／周，共４次，末次注射后１周处死，取肺脏常规切片，Ｈ．Ｅ染色，镜下观察病理变化，并通过５个系列浓度（０、５０、１００、２００和５００μｇ／ｍｌ）的ＳｉＯ２刺激ＴＨＰ－１细胞培养上清液作用于ＣＨＬ细胞（中国仓鼠肺成纤维细胞），采用四唑盐（ＭＴＴ）比色法检测ＣＨＬ细胞的增殖。结果：ＳｉＯ２刺激ＴＨＰ－１细胞培养上清液可引起大鼠肺内早期矽结节样病变。经ＭＴＴ分析，ＣＨＬ细胞增殖活性随着ＳｉＯ２剂量的增大而逐渐增强，呈剂量－效应关系。结论：ＳｉＯ２刺激的ＴＨＰ－１细胞上清液在体内可引起早期矽结节样病变。

Objective To study whether SiO 2 stimulated THP 1 cell(a monocyte like cell line with properties of the pulmonary alveolar macrophage)supernatants result in silicotic node like lesion.Methods The pathological changes of the lung of rats after intratracheal injection of 0 5ml of the supernatant one time/week×4.MTT assay to detect proliferation of CHL cells was carried after induced by THP 1 cells supernatants cultured for 36 hours in five different concentrations of silicon dioxide.Result The early silicotic node like lesion in the lung of rats were seen in the lung tissue of the rats after intratracheal injection of SiO 2 stimulated THP 1 cell supernatants.A 570mm values of MTT assay of CHL cells increased gradually along with the serial concentrations of silica and were fitted wll in the dose effect relationship.Conclusion The silica stimulated THP 1 cell supernatants can result in early silicotic node like lesion in the lung of rats and in vitro in crease CHL cells proliferative activity.