离子通道镶嵌点过程模型在K^+通道研究中的应用

Application of Embedded Point Process Model for Study on K + Channels

目的：用作者提出的离子通道镶嵌点过程模型拟合海马锥体细胞钾离子单通道记录数据分析１７β雌二醇的效应。方法：用协方差密度度量通道记忆，从３个方面做拟合优度检验。结果：该模型与观察资料拟合效果良好；１７β雌二醇能改变海马锥体细胞钾离子单通道的记忆；１７β雌二醇对关闭的影响比对开放的影响大，从而表现为对通道具有抑制作用：浓度为１０ｎｍｏｌ／Ｌ组的１７β雌二醇对通道记忆的影响比对浓度为０１ｎｍｏｌ／Ｌ组的大。结论：Ｍａｒｋｏｖ模型的常规分析只能得到１７β雌二醇对海马锥体细胞钾离子单通道具有抑制作用。

Objective: The model of stochastic point processes for ion channels proposed by the authors is used to fit the data recorded from the potassium channels in hippocampal neurons, and to analyze the effect of 17 β estradiol. Methods: Covariance density of point processes is applied to assess quantitatively the memory in ion channels and three methods are applied for the goodness of fit test. Results: The goodness of fit test shows that the model is fit; 17 β estradiol can change the memory of the potassium channels in hippocampal neurons; the effect of 17 β estradiol on close is greater than that on open, and hence it shows an inhibitory effect; the effect of 10 nmol/L 17 β estradiol on memory is greater than that of 0.1 nmoL/L. Conclusion: Applying Markov model with routine method, we can only conclude the effect of 17 β estradiol is an inhibitory one. While on the basis of the point process model, more information can be obtained than it.