电针治疗前后帕金森病模型大鼠纹状体D_2受体与多巴胺含量的对比研究

Comparative study of D 2 receptors and content of DA in striatum before and after electro acupuncture treatment

目的研究经电针治疗前后偏侧帕金森病（ＰＤ）模型大鼠纹状体多巴胺（ＤＡ）Ｄ２受体的变化，并将其与ＤＡ及其代谢产物的含量进行对比分析。方法用１２５Ｉ３碘２羟基６甲氧基Ｎ［（１乙基２吡咯烷）甲基］苯酰胺（ＩＢＺＭ）脑Ｄ２受体放射自显影、高效液相电化学检测器（ＨＰＬＣＥＣＤ）检测治疗前组、电针治疗组及治疗空白对照组偏侧ＰＤ模型大鼠纹状体ＤＡ及其代谢产物高香草酸（ＨＶＡ）、３，４二羟苯乙酸（ＤＯＰＡＣ）含量。结果电针治疗组患侧纹状体ＤＡ、ＨＶＡ、ＤＯＰＡＣ含量较治疗前组及治疗空白对照组增高（Ｐ＜００５）；电针治疗组患侧纹状体／小脑１２５ＩＩＢＺＭ摄取比值为８０４±０７１，较健侧升高（２９３４±４８３）％，与治疗前组的（８０９±０５２）相比，差异无显著性（Ｐ＞００５），但低于治疗空白对照组的（８６１±０６３），此值较健侧升高（３８６３±３７１）％。结论电针治疗可以提高大鼠实验性偏侧ＰＤ模型纹状体ＤＡ含量。

Objective To evaluate the change of D 2 receptors and its relationship with DA content in experimental hemi parkinsonism rats before and after electro acupuncture treatment Methods 125 I IBZM D 2 receptor cerebral autoradiographic analysis, HPLC ECD DA and its metabolites, homovanillic acid (HVA), 3,4 dihydroxyphenylacetic acid (DOPAC) content detection were used to study in striatum in before treatment, electro acupuncture treatment and treatment control group Results ①The DA, HVA and DOPAC level in striatum of lesioned side in electro acupuncture group was increased comparing with the before treatment and treatment control group (P<0 05) ②For the electro acupuncture group, the striatum/cerebellum 125 [KG*8/9]I IBZM uptake ratio was 8 04±0 71, (29 34±4 83)% more than that of the contralateral side, but no significant difference was observed as compared with that of the pretreatment group [(8 09±0 52), P>0 05]; however it was much lower than that of the treatment control group (8 61±0 63), P<0 05, and the latter was (38 63±3 71)% higher than that of the contralateral side, P<0 05 Conclusions Electro acupuncture treatment could increase the lesioned side striatum DA level to some extent, and can prevent D 2 receptors' upregulation in rats with experimental hemi parkinsonism