摘要
采用MTT法、碱性磷酸酶活性测定、油红O的染色和定量测定、矿化功能的测定以及qRT-PCR等手段在细胞和分子水平上研究了DyCl3对原代培养的成骨细胞增殖、分化和矿化功能的影响。研究结果表明,在测试浓度范围内,DyCl3均抑制成骨细胞增殖。浓度为1×10-8,1×10-6和1×10-5mol·L-1的DyCl3促进成骨细胞分化。在测试浓度范围内,DyCl3均抑制成骨细胞横向分化为脂肪细胞。浓度为1×10-5mol·L-1的DyCl3促进成骨细胞矿化结节的形成,而浓度为1×10-7mol·L-1和1×10-6mol·L-1的DyCl3抑制成骨细胞矿化结节的形成,进一步降低浓度为1×10-8mol·L-1,它则对成骨细胞矿化功能没有影响。浓度1×10-6mol·L-1的DyCl3显著降低PPAR-γmRNA表达水平,但相同浓度DyCl3则显著上调RUNX-2mRNA表达水平。实验结果提示,DyCl3对体外培养的成骨细胞增殖、分化及矿化功能的影响与浓度和作用时间有关,而且,它们是影响其生物效应从毒性到活性,从损伤到保护,从上调到下调转变的关键因素。这些结果对深入理解稀土离子对骨代谢的影响具有重要的价值。
A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide(MTT) test,alkaline phosphatase(ALP) activity measurement,Oil Red O stain and measurement,mineralized function expression and quantitive real time RT-PCR(qRT-PCR) were employed to assess the effects of DyCl3on the the proliferation,differentiation and mineralization function of primary osteoblasts(OBs) in vitro at cell and molecular levels.The results showed that DyCl3 inhibited the proliferation of OBs at tested concentrations.As to the differentiation of OBs,DyCl3 promoted the differentiation of OBs at concentrations of 1×10^-8,1×10^-6,and 1×10^-5 mol· L^-1.DyCl3 suppressed the adipogenic transdifferentiation of OBs at tested concentrations.DyCl3 promoted the formation of mineralized matrix nodules of OBs at a higher concentration 1 ×10^-5 mol·L^-1,turned to inhibit the formation of mineralized matrix nodules of OBs at concentrations of 1×10^-7 and 1×10^-6 mol·L^-1,but had no effect on the formation of mineralized matrix nodules of OBs at a concentration of 1×10^-8 mol·L^-1.The expression of the mRNA for PPAR-γ was significantly down-regulated,the expression of the mRNA for RUNX-2 was up-regulated in the presence of 1×10^-6 mol·L^-1 DyCl3.Our experimental results suggest that the effects of DyCl3 on the proliferation,differentiation and mineralization function of OBs in vitro depend on the concentration and culture time,moreover,they are pivotal factors for switching the biological effects of rare earth ions from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.These results are very helpful for elucidating deeply the effects of rare earth ions on bone metabolism.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2010年第10期1856-1862,共7页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金(No.20971034)
河北省自然科学基金(No.B2009000161)
教育部科学技术研究重点项目(No.208018)
河北省留学人员科技活动项目
河北大学自然科学基金资助项目
关键词
稀土离子
原代成骨细胞
增殖
分化
成脂横向分化
矿化
rare earth ions
primary osteoblasts
proliferation
differentiation
adipogenic transdifferentiation
mineralization