应用稀土调控藏红花胚性愈伤组织的生长与分化

Regulating the Cell Growth and Shoot Induction of Crocus sativus Embryogenic Callus by Rare Earth Elements

为提高藏红花(Crocus sativus)胚性愈伤组织的繁殖系数与出芽率,以建立藏红花离体快繁体系,解决其资源短缺问题,采用两步法,用稀土调控其胚性愈伤组织的生长与分化。结果表明,在添加了0.25mg·L-1NAA、3mg·L-16-BA和400mg·L-1CH的B5固体培养基中,0.04mmol·L-1La3+促进胚性愈伤组织生长的效果最佳,繁殖系数为12,是不添加稀土处理组的1.48倍;在添加了0.25mg·L-1NAA、3mg·L-16-BA和400mg·L-1CH的1/2B5固体培养基中,0.06mmol·L-1Ce3+促进胚性愈伤组织分化出芽的效果最佳,出芽率高达84.5%,是不添加稀土处理组的1.81倍,且高于国外报道的出芽率(40%)。初步解决了藏红花胚性愈伤组织生长慢和出芽率低等问题,为建立高效稳定的藏红花离体快繁体系奠定了基础。

Rare earth elements were used to promote the growth and induction of embryogenic callus of Crocus sativus by two-stage method for its micropropagation in vitro and then to solve the problems of its limited availability.For promoting cell growth,embryogenic calli were cultured on B5 solid medium supplemented with 0.25 mg·L-1 NAA,3 mg·L-1 6-BA,400 mg·L-1 CH,and 0.04 mmol·L-1 La3＋.The maximal proliferation rate was 12,which was 1.48-fold of that without rare earth elements.For promoting shoot induction,embryogenic calli were cultured on 1/2 B5 solid medium supplemented with 0.25 mg·L-1 NAA,3 mg·L-1 6-BA,400 mg·L-1 CH,and 0.06 mmol·L-1 Ce3＋.The maximal shoot induction rate was 84.5%,which was 1.81-fold of that without rare earth elements.In this study,rare earth elements could enhance the cell growth and shoot induction rate of embryogenic calli of C.sativus,which would be useful for its micropropagation.