联合检测P63、CK5诊断乳腺恶性乳头状病变的应用

The Concomitant application of P63 and CK5 detection for the diagnosis of malignant breast papillary lesions

目的:探讨通过免疫组化表型鉴别乳腺乳头状病变性质的应用价值。方法:回顾性分析2004年至2009年手术证实为乳腺乳头状病变病人的资料,分析肌上皮细胞相关标志物P63和CK5的表达,分别判定结果为阴性或阳性。结果:用P63阴性诊断恶性乳头状病变的灵敏度为90.90%,特异度为91.40%。使用CK5阴性诊断恶性乳头状病变,其灵敏度和特异度分别为84.85%和86.02%。使用P63和CK5双阴性诊断恶性乳头状病变的灵敏度和特异度分别为84.85%和100%。结论:联合检测P63和CK5可用于鉴别不确定的乳头状病变,当出现双阴性时考虑为恶性病变。

Objective This article aims to phenotyping by immunohistochemistry to identify the precise nature of the breast papillary lesions.Methods Retrospective analysis of the cases admitted from 2004 to 2009 undergoing surgical excision in patients with papillary lesions.Analysis of the myoepithelial cell-related markers,P63 and CK5 were identified as negative or positive.Results By using the P63 parameter,the sensitivity of its negative expression was 90.90%,and the specificity was 91.40%.CK5-negative could also be used to diagnose the malignant papillary lesions,the sensitivity and specificity were 84.85% and 86.02%.When using concomitantly the P63 and CK5 double-negative to diagnose the malignant papillary lesions,the sensitivity and specificity were 84.85% and 100%.Conclusions Detection of P63 and CK5 can be used to identify uncertain papillary lesions,when they are double-negative,the lesions can be considered as malignancy.