摘要
目的:探讨细胞周期蛋白D1(CyclinD1)、P16与增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)在子宫内膜异位症发生发展中作用。方法:应用SABC免疫组化技术检测子宫内膜异位症患者(观察组)在位内膜(25例)及异位子宫内膜(28例)和其它良性病变妇女子宫内膜组织(对照组,35例)中cyclinD1、P16及PCNA蛋白表达水平。结果:PCNA蛋白在异位内膜阳性表达均明显高于在位内膜及对照组(P<0.05);CyclinD1蛋白在异位内膜中阳性表达明显高于在位内膜和对照组(P<0.05);P16蛋白在对照组中阳性表达均明显高于在位内膜及异位内膜(P<0.05)。CyclinD1和P21蛋白在异位内膜中表达呈显著负相关性(kappa系数为-0.521,P<0.01)。三者在异位内膜增生期与分泌期表达无显著差异性(P>0.05)。结论:cyclinD1、P21及PCNA蛋白异常表达在子宫内膜异位症异位内膜增殖中起重要作用。
Objective：To investigate the possible roles of CyclinD1,P16 and PCNA play in the pathogenesis and development of endometriosis.Methods：Twenty-five specimens of eutopic endometrium and Twenty-eight specimens of ectopic endometrium tissues were obtained from 28 patients with endometriosis（observation group）.Thirty-five specimens of endometrium were also obtained from women with other benign diseases（control group）.CyclinD1,P16 and PCNA was detected by immunohistochemistry.Results：The positive expression of PCNA protein in ectopic endometrium was significantly higher than that in the eutopic endometrium and control group（P0.05）.The positive expression of CyclinD1 protein in ectopic endometrium was significantly higher than that in the eutopic endometrium and control group（P0.05）.The positive expression of P16 in control group was significantly higher than that in the eutopic endometrium and ectopic endometrium（P0.05）.It showed that in the ectopic endometrium the positive expression of CyclinD1 and P16 were negatively spearman correlation（kappa coefficient =-0.521,P0.01）.The positive expression of CyclinD1,P16 and PCNA protein in proliferative phase compared with the secretory phase of the eutopic endometrium,there was no significant difference（P0.05）.Conclusion：The abnormality expression of CyclinD1、P16 and PCNA in the ectopic endometrium may be involved in the proliferation of endometrium in endometriosis.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第9期1309-1312,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金资助(编号:30772034)
重庆市科委计划项目(编号:2007BB5316)
