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葡萄糖调节蛋白78对胃癌SGC7901细胞侵袭和转移的影响 被引量：2

Effect of GRP78 on invasion and metastasis of human gastric carcinoma SGC7901 cells in vitro
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摘要 目的:探讨钙离子螯合剂BAPTA-AM和钙离子载体A23187对胃癌SGC7901细胞葡萄糖调节蛋白78(Glucose regulated protein,GRP78)表达水平的影响,分析GRP78表达改变对胃癌细胞侵袭转移能力的影响。方法:BAPTA-AM和A23187处理细胞,RT-PCR和Western-blot检测3组细胞GRP78在mRNA和蛋白水平的表达,体外侵袭实验和划痕实验检测3组细胞的侵袭转移能力。结果:与对照组相比,BAPTA-AM组GRP78在mRNA和蛋白水平表达量降低,A23187组GRP78表达量升高;且BAPTA-AM组侵袭和转移能力明显低于对照组,而A23187组则高于对照组。结论:BAPTA-AM和A23187可分别下调和上调胃癌细胞GRP78的表达水平,GRP78的表达水平可能与胃癌细胞的侵袭和转移有关。 Objective：To investigate the effect of BAPTA-AM and A23187 on GRP78 expression of gastric carcinoma SGC-7901 cells in vitro,and the effect of GRP78 on invasion and metastasis of these tumor cells.Methods：The SGC7901 cells were treated by BAPTA-AM and A23187.RT-PCR and Western blot were used to assess the expression of GRP78 at both mRNA and protein levels.Matrigel invasion assay and a scratch wound assay were used to assess the invasion and migration levels.Results：The expression of GRP78 at both mRNA and protein levels,the invasion and migration levels were decreased in the BAPTA-AM-treated cells and increased in A23187-treated cells as compared with those of control group.Conclusion：BAPTA-AM can down-regulate the expression of GRP78 in SGC7901 cells and A23187 can up-regulate it.The expression of GRP78 in SGC7901 cells may be associated with their migration and invasion levels.
作者 颜晓丽 高青 李禄椿
机构地区 重庆医科大学附属第一医院消化内科
出处 《重庆医科大学学报》 CAS CSCD 北大核心 2010年第9期1332-1335,共4页 Journal of Chongqing Medical University
关键词 胃癌 葡萄糖调节蛋白78 BAPTA-AM 侵袭 转移 Gastric carcinoma GRP78 BAPTA-AM Invasion Metastasis
分类号 R735 [医药卫生—肿瘤]
  • 相关文献

参考文献14

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二级参考文献13

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共引文献14

同被引文献26

  • 1Ahn JH, Yu HK, Lee HJ, et al. Suppression of colorectal cancer liver metastasis by apolipoprotein (a) kringle V in a nude mouse model through the induction of apoptosis in tumor-associated endothelial cells. PLoS One, 2014, 9(4): e93794.
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引证文献2

二级引证文献3

重庆医科大学学报
2010年 第9期

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