阿奇霉素对胃内压和胃电的影响

Effect of azithromycin on intragastric pressure and gastric electrical activity

目的:观察静脉注射阿奇霉素(Azithromycin,AZM)对大鼠胃内压和胃电慢波的影响,并探讨阿奇霉素作用的神经和受体。方法:选用成年SD大鼠48只进行实验。颈静脉注射不同剂量的阿奇霉素或生理盐水(1ml),观察胃内压和胃电慢波变化(30只);先注射阿托品,再注射大剂量阿奇霉素观察胃内压变化(6只);膈下迷走神经切断后,注射大剂量阿奇霉素观察胃内压和胃电变化(12只)。结果:静脉注射中剂量(75mg/kg)和大剂量(150mg/kg)阿奇霉素后,大鼠胃内压增加,给药20min中剂量变化率有显著性(P<0.05),而大剂量出现变化早,在10min出现;两种剂量对胃慢波电位幅度和频率均无明显影响。使用阿托品后,阿奇霉素对胃内压的影响几乎完全阻断。膈下迷走神经切断后,阿奇霉素对胃内压作用被反转,胃内压下降明显,而对胃电无明显影响。阿奇霉素对空腹胃电改变不明显。结论:静脉注射阿奇霉素可增加胃内压,该效应可能通过迷走神经和胆碱能受体完成,阿奇霉素对胃电慢波幅度和频率可能无明显影响。

Objective：To observe the effect of azithromycin on intragastric pressure（IGP） and gastric electrical slow wave,and to explore the dependent nerve and receptor.Methods：Forty-eight SD rats were used for experiments.Animals received intravenous injection of different doses azithromycin or normal saline（1 ml）.Both IGP and gastric electrical activity were recorded by BL-410 Biological signal recording system（n=30）.Pretreatment of atropine was intraperitoneal injection,and then high-dose azithromycin was injected and IGP was recorded（n=6）.Pretreatment of vagotomy was carried out,and then high-dose was used and IGP and gastric electrical activity was recorded（n=12）.Results：Intravenous medium dose azithromycin（75 mg/kg） and high dose azithromycin（150 mg/kg） caused the increase of IGP,the change rate of IGP induced by medium dose had significance after 20 minutes,the change of high dose appeared at the 10th min.Different doses had no obvious influence on gastric electrical slow wave amplitude and frequency.No obvious changes in both IGP and gastric electrical activity was observed in normal saline.Atropine almost completely blocked the effect of azithromycin on IGP.Pretreatment of subdiaphragmal vagotomy affected the increase of IGP induced by azithromycin,IGP discreased obviously,but gastric electrical activity were not changed.Conclusion：Intravenous azithromycin could increase IGP,this effect might rely on vagus and cholinergic receptor;Azithromycin might have no obvious effect on gastric electrical slow wave amplitude and frequency.