应用组织芯片检测Pin1在肝炎、肝硬化、肝细胞性肝癌组织中的表达

Expression of the Pin1 in the hepatitis,cirrhosis and hepatocelluar carcinoma tissues and its relationship with hepatitis B virus by tissue microarray

目的:利用组织芯片技术检测肽基脯氨酰顺反异构酶(Peptidyl-proplyl isomerase1,pin1)在正常、肝炎、肝硬化及肝癌组织中的表达,了解pin1在肝细胞性肝癌发生、发展不同阶段的表达情况及与临床病理参数的关系。方法:用免疫组织化学染色法在组织芯片上同时检测10例HBsAg阳性病毒性肝炎、40例肝硬化、19例肝细胞性肝癌及34例正常肝组织中pin1的表达情况,并分析pin1在不同肝脏疾病组织中的表达差异。结果:pin1在HBsAg阳性病毒性肝炎、肝硬化、肝细胞性肝癌及正常肝组织中的阳性表达率分别为80%(8/10)、82.5%(33/40)、100%(19/19)、55.8%(19/34),肝细胞性肝癌组织中的阳性表达率明显高于HBsAg阳性病毒性肝炎、肝硬化及正常肝组织,同时肝癌中pin1的表达强度也明显高于HBsAg阳性病毒性肝炎、肝硬化和正常肝组织(P<0.001),而在HBsAg阳性病毒性肝炎、肝硬化和正常肝组织中pin1阳性表达率及表达强度均无差异无统计学意义(P>0.001);肝细胞性肝癌组织中pin1的表达与病理分级、有无淋巴结转移及临床分期无相关性(P>0.05)。结论:pin1参与了HBsAg阳性病毒性肝炎,肝硬化到肝细胞性肝癌的疾病进展过程,在肝细胞性肝癌的发生和发展中发挥了一定作用。

Objective：By using tissue microarray technique to detect the expression of peptidyl-proplyl isomerase 1（pin1） in human hepatitis B,cirrhosis,hepatocelluar carcinoma（HCC） and normal liver tissue,discussing its role in different stage of oncogenesis of HCC,and its relationship with clinicopathological parameter.Methods：Immunohistochemistry was used to detect the expression of pin1 in 10 cases of hepatitis B,40 cases of cirrhosis,19 cases of HCC and 34 cases of normal liver tissue.Results：The positive expression rate and intensity of pin1 was stronger in the HCC than that in the hepatitis B,cirrhosis and normal liver tissue（P0.001）,but there was no significant difference in the latter three tissues（P0.001）.The expression of pin1 was not correlated with clinicopathological parameter（P0.05）.Conclusion：pin1 plays a pivotal role in different stages of HCC.