大鼠骨骼肌挫伤后p-CB1R的表达及与损伤时间的相关性

The time-dependent expression of p-CB1R during repair of skeletal muscle contusion in rats

目的观察探讨大鼠骨骼肌挫伤愈合过程中p-CB1R的表达规律与损伤时间的相关性。方法健康成年雄性SD大鼠50只,体重220～250g,随机分为10组,每组5只,其中9组为实验组,1组为正常对照组。应用免疫组织化学染色和Western blot技术,检测50例大鼠骨骼肌挫伤后各时间段p-CB1R的表达情况。结果正常对照组大鼠骨骼肌未见p-CB1R表达;挫伤后p-CB1R阳性表达主要在单核细胞和成纤维细胞中,各时间段大鼠骨骼肌均未见阳性反应。伤后3～24h,阳性细胞以单核细胞为主;3～5d,阳性细胞以单核细胞和成纤维细胞为主;7～10d,阳性反应主要见于成纤维细胞,并于7d达到高峰;14d,p-CB1R阳性表达量有所下降。结论骨骼肌挫伤愈合过程中p-CB1R在单核细胞和成纤维细胞中的变化具有时序性,可望作为法医学推断骨骼肌挫伤时间的参考指标之一。

Objective To investigate the expression of p-CB1R during wound healing of skeletal muscle contusion in rats and to explore the applicability of p-CB1R to wound age estimation.Methods A total of 50 male,healthy rats,weighting 220～250g were divided into 10 groups randomly.The changes of p-CB1R expression were assessed by immunostaining and Western blotting.Results The expression of p-CB1R was neither detected in skeletal muscle fibers in control rats nor wounded skeletal muscle.No-immunoreactivity of p-CB1R was detected in polymorphonuclear cells（PMNs） in the wound specimens.p-CB1R-positive immunostaining was detected in mononuclear cells（MNCs） in the wounded specimens aged from 3h to 1d.From 3d to 5d postwounding,p-CB1R-immunoreactivity was mainly found in MNCs and fibroblastic cells（FBCs）,and mainly in FBCs from 7d to 10d postwounding with a peak at the 7d and then decreased at 14d.A similar tendency for p-CB1R protein changes was noted as determined by Western blotting.Conclusion p-CB1R is time-dependently expressed in MNCs and FBCs during wound healing of skeletal muscle contusion in rats,suggesting that it may be used as a marker for wound age determination.