清营泻瘀方对肠缺血再灌注损伤大鼠肠屏障的保护作用

Protective effect of Qing Ying Xie Yu Fang(清营泻瘀方) on Gut Barrier Function on Intestine Ischemia-Reperfusion Injury in Rats

目的:探讨清营泻瘀方对肠缺血再灌注损伤大鼠肠屏障的保护作用及可能机制。方法:80只SD大鼠,随机分为假手术组(n=8)、肠缺血再灌注模型组(n=24)、清营泻瘀方治疗组(n=24)和大承气方治疗组(n=24),后3组按造模后处死时间的不同又分别分为术后2h组、术后24h组和术后48h组,每组8只。采用夹闭肠系膜上动脉45min的方法诱导肠缺血再灌注损伤模型,分别观察再灌注后2h、24h和48h肠黏膜的病理改变以及血清中TNF-α、IL-10和血浆中D-乳酸的水平。结果:清营泻瘀方和大承气方都能够减轻肠黏膜的损伤,降低血中TNF-α和D-乳酸的含量(P<0.05),晚期降低IL-10水平(P<0.05)。清营泻瘀方在在保护肠黏膜和降低血D-乳酸的水平方面优于大承气方(P<0.05)。结论:清营泻瘀方对肠缺血再灌注损伤大鼠肠屏障有显著的保护作用,其可能机制与荡涤肠胃宿垢,减少细菌移位、调节炎症介质平衡有关。

Objective To study the effects and mechanism of Qing ying xie Yu Fang （QXF）（清营泻瘀方）in the protection of gut barrier function of intestinal ischemia-reperfusion in rats. Methods Eighty Sprague Dawley rats were randomly divided into sham （n=8）, model （n=24）, QXF （n=24） and Da cheng Qi Fang （CQF）（大承气方） （n=24） groups. The last three groups were respectively divided into 2 hour group, 24 hour group and 48 hour group. Intestinal isxhemia-reperfusion model in rats was induced by clamping the superior mensenteric artery for 45 minutes period. The pathologic injury of the intestine and the level of TNF-α, IL-10, D-lactic acid in blood were examined at 2 hour point, 24 hour point and 48 hour point after reperfusion respectively. The statistical analysis was finished with SPSS11.0 software. Results QXF and CQF both alleviated the pathologic injury of the intestine and reduced the levels of TNF-α,D-lactic acid （P,0.05） in the early period and IL-10 （P0.05） in the late period. But QXF had better curative effects than CQF in reducing the level of D-lactic acid （P0.05）. Conclusion QXF can reduce the gut injury of intestinal ischemia-reperfusion with the following possible mechanism： to clear up dunghill in intestine, regulate the balance of inflammatory mediators and protect gut barrier function.