碱性成纤维细胞生长因子对牙周膜成纤维细胞整合素β1亚单位mRNA表达的影响

The effect of basic fibroblast growth factor on the mRNA expression of β1 integrin subunit by periodontal ligament fibroblasts in culture

目的研究碱性成纤维细胞生长因子(bFGF)对人牙周膜成纤维细胞整合素β1亚单位mRNA表达的影响,探讨bFGF在牙周组织再生中的意义。方法体外培养人牙周膜成纤维细胞,分别用质量浓度0.1、1.0、10.0 ng.mL-1的bFGF刺激细胞,培养24、48、72 h,采用实时荧光定量聚合酶链反应法检测牙周膜成纤维细胞内整合素β1亚单位mRNA表达的变化。结果 bFGF可促进人牙周膜成纤维细胞内整合素β1亚单位mRNA的合成,在培养24、48、72 h时,1.0 ng.mL-1组整合素β1亚单位表达均明显高于对照组;培养72 h时各实验组整合素β1亚单位表达均明显高于培养24、48 h时。结论 bFGF通过提高整合素β1亚单位mRNA的表达,促进牙周膜成纤维细胞的黏附,在牙周组织修复再生中起作用。

Objective To study the effect of basic fibroblast growth factor on the mRNA expression of β1 integrin subunit by periodontal ligament fibroblasts in culture;to discuss the effect of basic fibroblast growth factor in periodontal regeneration.Methods Human periodontal ligament fibroblasts were cultured and stimulated by basic fibroblast growth facto（r0.1,1.0,10.0 ng.mL-1） for 24,48,72 h respectively,and then mRNA expression of β1 inte-grin subunit was assessed by fluorescent quantitative real-time reverse transcription polymerase chain reaction.Results Basic fibroblast growth factor enhanced the mRNA expression of β1 integrin subunit,and there was optimal effect when the concentration of basic fibroblast growth factor was 1.0 ng.mL-1 at 24,48,72 h respectively;the mRNA expression of β1 integrin subunit at 72 h was higher than that at 24,48 h.Conclusion Basic fibroblast growth factor can strengthen human periodontal ligament fibroblasts′ adhesion and may be one of important factors which participate in the periodontal regeneration.