低强度脉冲超声波对Beagle犬牙槽骨缺损的修复效应

Repairing effects of low-intensity pulsed ultrasound on alveolar bone defects in Beagle dogs

目的利用低强度脉冲超声波(LIPUS)辐照Beagle犬下颌前磨牙水平型牙槽骨缺损模型,探讨其促进急性牙槽骨缺损修复的潜在效应。方法选用4只Beagle犬的下颌双侧第三、四前磨牙颊侧区,制备釉牙骨质界下6 mm深的水平型牙槽骨缺损模型。左右两侧随机分为实验组和对照组,实验组采用LIPUS辐照(ISATA 30 mW·cm-2,20 min·d-1),对照组为不开功率源的假辐照。辐照8周后,使用双能X线骨密度仪检测新生牙槽骨骨密度,脱钙骨组织切片观察新生牙槽骨的组织学效应。结果实验组与对照组新生牙槽骨骨密度分别为(0.605 3±0.056 6)g·cm-2、(0.604 7±0.055 2)g·cm-2,2组之间的差异无统计学意义(P=0.983 9)。脱钙骨组织切片苏木精-伊红染色显示实验组新生牙槽骨周边成骨细胞成排排列,数量较多,而对照组成骨细胞散在分布,数量少;Masson染色示实验组新生骨组织中胶原呈鲜红色,成熟度高,而对照组以蓝色为主,可见绿色区域,成熟度偏低。结论 LIPUS辐照对急性水平型牙槽骨缺损具有潜在的修复效应。

Objective To evaluate the potential repairing effects of low-intensity pulsed ultrasound（LIPUS） irradiation on acute horizontal alveolar bone defects at the mandibular pre-molar areas in Beagle dogs.Methods Horizontal alveolar bone defect models were established under enamelo-cemental junction 6 mm at the mandibular third and forth pre-molar buccal regions on both sides in 4 beagle dogs,and bilateral sides of each dog were randomly divided into two groups： Experimental groups with LIPUS irradiation（ISATA 30 mW.cm-2,20 min.d-1） and control groups without opening power source of LIPUS radiation.Dual energy X-ray bone densitometer was used to detect the bony density after an 8 weeks′ irradiation.Meanwhile,decalcified bone tissue sections were used to assess the histological effects of new alveolar bone.Results The results of new bony density detection in experimental group and control group were（0.605 3±0.056 6） g.cm-2,（0.604 7±0.055 2）g.cm-2,respectively,and there was no statistical significance between the differences of the two groups（P=0.983 9）.Hematoxylin-eosin staining of decalcified bone tissue sections demonstrated that there were more osteoblasts lining at the edge of new alveolar bone in the experimental groups than that scattered in the control groups,and Masson staining revealed that collagens in new alveolar bone stained bright red indicating higher maturity in the experimental groups,while in the control groups mainly stained blue with some virescent areas indicating lower maturity.Conclusion LIPUS irradiation on acute horizontal alveolar bone defects has potential repairing effects.