龙眼24个品种的SCoT遗传多样性分析

Analysis on the Genetic Diversity of 24 Longan (Dimocarpus longan) Accessions by SCoT Markers

利用SCoT标记对24份龙眼品种资源进行了检测。从80条引物中筛选出24条重复性好,条带清晰的引物进行PCR扩增,共扩增出211条带,其中181条具有多态性,多态性带比率为85.8%。24个龙眼品种间遗传相似系数在0.65～0.86之间,说明SCoT标记能够揭示材料间较高的遗传多样性。UPGMA聚类分析显示,SCoT标记能将24份龙眼品种完全区分开,并分成两类。

SCoT markers were used to study genetic diversity and relationship of 24 longan accessions.Twenty-four primers were screened from 80 SCoT primers and then used to amplify the genome DNAs of the accessions.Totally 211 SCoT loci were produced,including 181 polymorphic loci,with a polymorphism rate of 85.8%.The Jaccard genetic similarity coefficients among the accessions were calculated with NTSYS-pc2.10e software and the values were between 0.65 and 0.86.The results showed that there was relatively high genetic diversity among the accessions.The cluster analysis conducted with UPGMA showed that 24 accessions could be distinguished from each other and divided into two groups.