大孔吸附树脂分离纯化元宝枫叶总多酚

Separation and Purification of Total Polyphenols from Acer truncatum Bunge Leaves with Macroporous Adsorption Resin

目的研究大孔树脂分离纯化元宝枫叶总多酚的工艺条件。方法以元宝枫叶总多酚的静态与动态吸附量和解吸率为指标筛选大孔树脂,采用紫外分光光度法测定元宝枫叶总多酚含量,对所筛选树脂分离元宝枫叶总多酚工艺进行评价。结果H PD100型大孔树脂分离效果较好,其最佳分离条件是以浓度为5 g/L元宝枫叶提取物为供试液,上样和洗脱流速为2 mL/min,解吸液浓度为70%乙醇,解吸液体积为4BV。结论HPD100型大孔树脂在所确定的工艺条件下,树脂的吸附-解吸附性能稳定,且能较好地分离纯化元宝枫叶总多酚,多酚含量纯化前为28.55%,纯化后为39.52%。

Objective To study the optimal techniques for separation of total polyphenols from the leaves of Acer truncatum bunge with macroporous adsorption resins.Methods The static and dynamic adsorption-desorption characteristics were taken as index to select the macroporous adsorption resin.The separating efficiency was evaluated by measuring the concentration of total polyphenols in A.truncatum.with UV spectrophotometer.Results The HPD100 macroporous adsorption resin had the best separating efficiency.The optimum separation conditions of macroporous adsorption resin HPD100 are as follows： the concentration of the extract from A.truncatum.leaves solution is 5 mg/mL,and the velocity is 2 mL/min,with 4BV of 70% ethanol to elute.Conclusion The total polyphenols in the leaves of A.truncatum was able to be easily separated and purified by HPD100 resin,and its adsorption-desorption characteristics were stable under the optimum conditions mentioned above.The original content of polyphenols was 28.55%,after purification with HPD100,it could reach 39.52%.