内皮型一氧化氮合酶基因多态性与缺血性脑血管病后非痴呆认知功能障碍的关系

Association of endothelial nitric oxide synthase gene polymorphism with vascular cognitive impairment no dementia after ischemic cerebral disease

目的探讨内皮型一氧化氮合酶(eNOS)基因多态性与缺血性脑血管病后血管性非痴呆认知功能障碍(V-CIND)的相关性。方法采用PCR-RFLP技术对100例缺血性脑血管病后V-CIND患者,80例缺血性脑血病患者认知功能正常患者(NoCI)和120例正常人的eNOS G894T和eNOS 4a的多态性进行测定。结果三组在eNOS G894T的基因型分布和等位基因的频率方面有统计学差异,V-CIND和NoCI含T的等位基因频率都高于对照组,且具有统计学意义,但此两者之间没有区别;三组在eNOS4a的含a等位基因的频率有差异,但无统计学意义。结论 eNOS G894T基因多态性与缺血性脑血管病变后V-CIND相关,T等位基因可能是其易感基因。

Objective To study the relationship between endothelial nitric oxide synthase（eNOS）gene polymorphism and vascular cognitive impairment no dementia（V-CIND） after ischemic cerebral disease.Methods 180 patients with V-CIND after ischemic cerebral disease were divided into no cognitive impairment（NoCI） and V-CIND groups.They were all received cognitive assessment,such as MMSE, CDR and CSE-D,and then a total of 120 healthy people from the Health Management Center was recruited as control group（CR）.Polymerase chain reaction combined with restrictive enzyme digestion was used to detect the G894T polymorphisms and 4a polymorphisms of eNOS gene in patients.Results There were significant differences in eNOSG894T genotype distribution and T allele frequency among V-CIND, NoCI and CR.Patients with V-CIND showed a significantly higher frequency of the T allele compared to the CR（19% vs 8.9%,χ2=10.301,P0.010,OR=1.73）,patients with NoCI also showed a significantly higher frequency of the T allele compared to the CR（18.75% vs 8.9%,χ2=10.731,P0.010,OR=2.68）,there was no difference in frequency of the T allele between V-CIND and NoCI.As to the frequency of the a allele,there were differences among three groups,but they had no statistical significance.Conclusions The G894T mutation of eNOS is related with V-CIND ischemic cerebral disease,T allele may be the susceptibility gene.