用单倍体相合小鼠FBL-3红白血病细胞株移植建立CB6F1小鼠红白血病模型

Establishment of A Erythroleukemia Model in CB6F1 Mice by Transplant with Haploidentical Mouse Leukemic Cell Line FBL-3

本研究探讨建立F1代单倍体相合小鼠FBL-3红白血病模型的可行性,并观察FBL-3细胞在小鼠体内的生物学特性。将FBL-3H2-d小鼠红白血病细胞经尾静脉分别接种给小鼠C57BL/6和CB6F1H-2b/d(C57BL/6×BALB/c),观察两种小鼠的生存时间和染色体变化,并对濒死小鼠的肝、脾、肺和肾进行病理检查,部分进行电子显微镜检查。分析骨髓和脾脏细胞染色体核型及MHC分子表达情况。结果表明:静脉接种103-107个白血病细胞的情况下,C57BL/6小鼠和CB6F1小鼠发病率分别为100%和92.5%;接种的细胞数量与存活时间呈线性关系;接种相同数量FBL-3细胞的CB6F1小鼠平均生存时间较C57BL/6小鼠延长。白血病细胞主要侵及肝、脾、骨髓、肺和肾组织,糖原染色阳性、氯醋酸染色部分阳性,过氧化物酶、碱性磷酸酶、丁酸染色均为阴性。电子显微镜观察到细胞内病毒样颗粒。脾脏和骨髓细胞染色体多数为非二倍体,且H-2b表达率升高,H-2d表达率降低。结论:经尾静脉接种FBL-3细胞可以建立CB6F1小鼠红白血病模型。

The purpose of study was to investigate the feasibility for establishing erythroleukemia model in CB6F1 mice by transplant with haploidentical mouse leukemic cell line FBL-3 and to explore the biological characteristics of FBL-3 cells in CB6F1 mice,CB6F1 and C57BL/6 mice were inoculated intravenously at doses of 1×103-1×107 FBL-3 cells respectively.The survival time,the count of peripheral white blood cells,the percentage of erythroblasts and chromosome of these mice were observed.The liver,spleen,lung and kidney were obtained from the dying CB6F1 mice for pathological examination.The ultrastructure of erythroblasts in bone marrow and spleen was observed by transmission electron microscopy as soon as these mice died.Expression of MHC molecules and karyotype of spleen and bone marrow cell were measured.The results showed that 100% and 92.5% incidences of erythroleukemia were observed when 1×103-1×107 FBL-3 cells had been administrated intravenously to CB6F1 and C57BL/6 mouse,respectively.There was a linear relationship between the survival time and the number of inoculated leukemic cells.The survival time of CB6F1 was longer than C57BL/6 mice inoculated the same number cell.The main targets for FBL-3 cell infiltration were liver,spleen,marrow,lung and kidney.The reaction of FBL-3 cells to glycogen staining was positive,while the to reaction peroxidase,alkaline phosphatase and butyric acid staining were negative,reaction to chloroacetic acid staining partially was positive.Virus-like particles were found in the spleen and bone marrow cells under electron microscope.Chromosomes of spleen and bone marrow cells in the majority were non-diploid,and the expression of H-2b increased,H-2d expression decreased.It is concluded that the erythroleukemia mouse model can be established in CB6F1 mice transplanted with leukemic FBL-3 cells,that provides a convenience experimental erythroleukemia model for study.