卡介苗对白血病患儿外周血树突状细胞扩增的影响

Effect of Bacillus Calmette-Guerin on the Expansion of Dendritic Cells from Peripheral Blood of Pediatric Patients with Leukemia In Vitro

本研究探讨卡介苗(bac illus calm ette-guerin,BCG)对白血病患儿外周血来源树突状细胞(dendritic cell,DC)体外扩增的影响。本实验分为2组,即对照组和实验组,后者分为三个亚组即BCG组(单纯卡介苗),GTI组(细胞因子组)和GTIB组(细胞因子+卡介苗组)。培养至第9天,对各组细胞进行计数,应用流式细胞术检测各组细胞免疫表型,并行瑞氏-姬姆萨染液染色,在油镜下观察各组细胞的形态。结果表明:①实验各组均得到一定数量典型的DC,其中BCG组DC数低于GTI组和GTIB组(t分别为4.20,6.36,p均<0.01),GTI组DC数与GTIB组DC数比较无显著差异(t=2.25;p>0.05);②BCG组CD1 a+细胞比例明显高于对照组(t=3.04,p<0.05),但低于GTI组和GTIB组(t分别为2.79,6.41,p均<0.05);GTI组与GTIB组CD1 a+细胞比例比较差异无显著性(t=0.65,p>0.05)。BCG组HLA-DR+、CD83+细胞比例亦明显高于对照组(t分别为4.77,4.15;p<0.05),而低于GTI组和GTIB组(t分别为6.65,3.19;p均<0.05),GTI组HLA-DR+、CD83+细胞比例低于GTIB组(t分别为5.64,2.98;p均<0.05)。结论:BCG不仅能促进白血病患儿外周血DC体外扩增,还能协同rhGM-CSF、rhTNF-α、rhIL-4促进DC成熟。

This study was purposed to investigate the effect of bacillus calmette-guerin（BCG） on the expansion of human dendritic cells（DC） from peripheral blood of pediatric patients with leukemia in vitro.The experiment was divi-ded into two groups： the control and the test group,and the latter group was divided into 3 subgroups： BCG（only BCG）,GTI（GM-CSF,TNF-α,IL-4） and GTIB（GM-CSF,TNF-α,IL-4 plus BCG）.On day 9 of culture the DCs were counted in each groups,the phenotypes of DC were determined by flow cytometry and these DC were stained with Wright-Giemsa for observation and photography under microscopy.The results showed that the test groups all obtained a certain amount of typical DC;the number of DC in the BCG subgroup is lower than that in the GTI and GTIB subgroups（t=4.20;6.36,p0.01）;there was no significant difference between the GTI and the GTIB subgroups（t=2.25;p0.05）.The rate of CD1a＋ in the BCG subgroup was obviously higher than that in the control group（t=3.04,p 0.05）,but was lower than that in the GTI and the GTIB subgroups（t=2.79,6.41,p 0.05）,there was no significant difference between the GTI and the GTIB subgroups（t=0.65,p 0.05）.The rate of HLA-DR＋,CD83＋ in the BCG group was higher than that in the control group（t=4.77,4.15;p 0.05）,but lower than that in the GTI and the GTIB subgroups（t=6.65,3.19;p0.05）.The rate of HLA-DR＋,CD83＋ in the GTI subgroup was lower than that in the GTIB subgroup（t=5.64,2.98;p0.05）.It is concluded that BCG not only promotes the proliferation of DC derived from human peripheral blood of leukemia patients in vitro,but also cooperates with rhGM-CSF,rhTNF-α and rhIL-4 in promoting the maturation of DCs.